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MicroRNA-1185 Induces Endothelial Cell Apoptosis by Targeting UVRAG and KRIT1
- Source :
- Cellular Physiology and Biochemistry, Vol 41, Iss 6, Pp 2171-2182 (2017)
- Publication Year :
- 2017
- Publisher :
- S. Karger AG, 2017.
-
Abstract
- Background/Aims: Atherosclerosis is a multifactorial chronic disease and is the main cause of death and impairment in the world. Endothelial injury and apoptosis play a crucial role in the onset and development of atherosclerosis. MicroRNAs (miRNAs) have been proven to be involved in the pathogenesis of atherosclerosis. However, studies of the functional role of apoptosis-related miRNAs in the endothelium during atherogenesis are limited. Methods: Cell injury and apoptosis were measured in five types of cells transfected with miR-1185 or co-transfected with miR-1185 and its inhibitor. Bioinformatics analysis and a luciferase reporter assay were used to confirm the targets of miR-1185. The effects of the targets of miR-1185 on endothelial apoptosis were determined using small-interfering RNA. Results: In this study, we first report that miR-1185 significantly promoted apoptosis in endothelial cells but not in vascular smooth muscle cells and macrophages. A mechanistic analysis showed that ultraviolet irradiation resistance-associated gene (UVRAG) and krev1 interaction trapped gene 1 (KRIT1), targets of miR-1185, mediated miR-1185-induced endothelial cell apoptosis. Conclusion: The results revealed the impact of miR-1185 on endothelial apoptosis, suggesting that miR-1185 may be a potential target for the prevention and treatment of atherosclerosis.
- Subjects :
- 0301 basic medicine
KRIT1
Endothelium
Physiology
Down-Regulation
Apoptosis
Caspase 3
UVRAG
Biology
lcsh:Physiology
lcsh:Biochemistry
03 medical and health sciences
Downregulation and upregulation
RNA interference
Proto-Oncogene Proteins
microRNA
Human Umbilical Vein Endothelial Cells
medicine
Humans
lcsh:QD415-436
RNA, Small Interfering
3' Untranslated Regions
KRIT1 Protein
lcsh:QP1-981
Base Sequence
MiR-1185
Tumor Suppressor Proteins
Antagomirs
Transfection
Atherosclerosis
Up-Regulation
Cell biology
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
Cancer research
RNA Interference
Microtubule-Associated Proteins
Sequence Alignment
Subjects
Details
- ISSN :
- 14219778 and 10158987
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- Cellular Physiology and Biochemistry
- Accession number :
- edsair.doi.dedup.....0148439ec81626fb7ddc7919eb52acd0