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Functional kaurene-synthase-like diterpene synthases lacking a gamma domain are widely present in Oryza and related species
- Source :
- Bioscience, Biotechnology, and Biochemistry. 85:1945-1952
- Publication Year :
- 2021
- Publisher :
- Informa UK Limited, 2021.
-
Abstract
- Various diterpene synthases have been functionally identified in cultivated rice (Oryza sativa). These are the homologs of ent-copalyl diphosphate (ent-CDP) synthase and ent-kaurene synthase (KS) that are responsible for the biosynthesis of gibberellins, diterpenoid phytohormones. We isolated a cDNA encoding full-length OsKSL12, a previously uncharacterized KS like (KSL) enzyme that consists of a β-domain and an α-domain with an active center, but lacks an N-terminal γ-domain. Functional analysis using a bacterial expression system showed that recombinant OsKSL12 converted ent-CDP into ent-manool or ent-13-epi-manool. Comparative genomics revealed that functional OsKSL12 homologs exist in diverse wild species in the Oryzeae—Oryza nivara (Oryza rufipogon), Oryza coarctata, Oryza granulata, Leersia perrieri, and Leersia tisseranti. KSL12 homologs in O. granulata, L. perrieri, and L. tisseranti preferentially reacted with geranylgeranyl diphosphate rather than ent-CDP, resulting in geranyllinalool rather than ent-manool or ent-13-epi-manool as the main product, meaning that KSL12 functionally diversified during evolution in the Oryzeae.
- Subjects :
- 0106 biological sciences
DNA, Complementary
education
Oryza
01 natural sciences
Applied Microbiology and Biotechnology
Biochemistry
Analytical Chemistry
Evolution, Molecular
Leersia
03 medical and health sciences
chemistry.chemical_compound
Protein Domains
Complementary DNA
otorhinolaryngologic diseases
Cloning, Molecular
Molecular Biology
Phylogeny
030304 developmental biology
0303 health sciences
Alkyl and Aryl Transferases
Oryza sativa
Base Sequence
ATP synthase
biology
Organic Chemistry
food and beverages
General Medicine
biology.organism_classification
Oryza rufipogon
Oryzeae
chemistry
biology.protein
Diterpenes
Diterpene
Genome, Plant
010606 plant biology & botany
Biotechnology
Subjects
Details
- ISSN :
- 13476947
- Volume :
- 85
- Database :
- OpenAIRE
- Journal :
- Bioscience, Biotechnology, and Biochemistry
- Accession number :
- edsair.doi.dedup.....015bd7d666c181fe4f9fccadd6a4b20e