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The heat shock protein 90 of Toxoplasma gondii is essential for invasion of host cells and tachyzoite growth
- Source :
- Parasite, Parasite, Vol 24, p 22 (2017)
- Publication Year :
- 2017
-
Abstract
- Toxoplasma gondii is an obligate intracellular apicomplexan parasite that infects almost all warm-blooded vertebrates. Heat shock proteins (HSP) regulate key signal transduction events in many organisms, and heat shock protein 90 (Hsp90) plays an important role in growth, development, and virulence in several parasitic protozoa. Here, we discovered increased transcription of the Hsp90 gene under conditions for bradyzoite differentiation, i.e. alkaline and heat shock conditions in vitro , suggesting that Hsp90 may be connected with bradyzoite development in T. gondii . A knockout of the Tg Hsp90 strain (ΔHsp90 ) and a complementation strain were constructed. The Tg Hsp90 knockout cells were found to be defective in host-cell invasion, were not able to proliferate in vitro in Vero cells, and did not show long-time survival in mice in vivo. These inabilities of the knockout parasites were restored upon complementation of Tg Hsp90. These data unequivocally show that Tg Hsp90 contributes to bradyzoite development, and to invasion and replication of T. gondii in host cells.
- Subjects :
- 0301 basic medicine
Hot Temperature
Antibodies, Protozoan
Fluorescent Antibody Technique
Gene Knockout Techniques
Mice
Invasion
Chlorocebus aethiops
Mice, Inbred BALB C
biology
Virulence
Hydrogen-Ion Concentration
Hsp90
Recombinant Proteins
Cell biology
Complementation
Infectious Diseases
Differentiation
Female
Rabbits
Signal transduction
Toxoplasma
Research Article
Radioimmunoprecipitation Assay
Veterinary (miscellaneous)
Blotting, Western
Toxoplasma gondii
Replication
Enzyme-Linked Immunosorbent Assay
Real-Time Polymerase Chain Reaction
lcsh:Infectious and parasitic diseases
03 medical and health sciences
Heat shock protein
parasitic diseases
Animals
lcsh:RC109-216
HSP90 Heat-Shock Proteins
Heat shock protein 90
Gene
Vero Cells
Genetic Complementation Test
biology.organism_classification
Virology
030104 developmental biology
Insect Science
biology.protein
Vero cell
Animal Science and Zoology
Parasitology
Subjects
Details
- ISSN :
- 17761042
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Parasite (Paris, France)
- Accession number :
- edsair.doi.dedup.....016e0d54968a37e2061546162d3cf85e