Interleukin-8 expression associated with canine mammary tumors

Made available in DSpace on 2013-09-27T14:53:47Z (GMT). No. of bitstreams: 1 WOS000295823500029.pdf: 870388 bytes, checksum: 49bad93d964916582d5623fb0c1d7e31 (MD5) Previous issue date: 2011-01-01 Made available in DSpace on 2013-09-30T19:24:29Z (GMT). No. of bitstreams: 1 WOS000295823500029.pdf: 870388 bytes, checksum: 49bad93d964916582d5623fb0c1d7e31 (MD5) Previous issue date: 2011-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:34:37Z No. of bitstreams: 1 WOS000295823500029.pdf: 870388 bytes, checksum: 49bad93d964916582d5623fb0c1d7e31 (MD5) Made available in DSpace on 2014-05-20T15:34:37Z (GMT). No. of bitstreams: 1 WOS000295823500029.pdf: 870388 bytes, checksum: 49bad93d964916582d5623fb0c1d7e31 (MD5) Previous issue date: 2011-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The use of prognostic markers for mammary cancer is important for routine diagnosis and research. Interleukin-8 (IL-8) is a chemotactic cytokine, produced by several cell types in response to inflammation. The expression, regulation and function of IL-8 in dogs are little known. Recent studies have associated angiogenesis and inflammatory processes with tumor malignancy. We investigated a possible correlation between IL-8 expression and mammary tumor prognosis in female dogs. IL-8 expression was measured in 50 dogs with mammary neoplasia by immunohistochemistry and real-time PCR. Immunohistochemical staining was done with anti-IL-8 antibodies and PCR amplifications were performed in a 7500 Fast Real-Time PCR system. Gene expression stability was analyzed by the geNorm software. Quantitative real-time PCR showed that IL-8 expression decreased in malignant mammary cells compared to normal mammary tissue, while weak immunostaining was associated with a diagnosis of carcinoma. Complementing earlier studies on IL-8 expression in several types of cancer, including mammary cancer, we conclude that IL-8 has potential for use as a prognostic marker for canine mammary neoplasia. Faculdade de Medicina de São José do Rio Preto (FAMERP), Programa Posgrad Ciencias Saude, Sao Jose do Rio Preto, SP, Brazil Univ Estadual Paulista, Programa Posgrad Genet, Sao Jose do Rio Preto, SP, Brazil Univ São Paulo, Inst Biociencias, São Paulo, Brazil Univ Estadual Paulista, Programa Posgrad Genet, Sao Jose do Rio Preto, SP, Brazil FAPESP: 05/51936-0