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Bacteria-derived long chain fatty acid exhibits anti-inflammatory properties in colitis
- Source :
- Gut, Gut, BMJ Publishing Group, In press, 70 (6), pp.1088-1097. ⟨10.1136/gutjnl-2020-321173⟩, Gut, Vol. 70, p. 1088-1097 (2021)
- Publication Year :
- 2021
- Publisher :
- HAL CCSD, 2021.
-
Abstract
- ObjectiveData from clinical research suggest that certain probiotic bacterial strains have the potential to modulate colonic inflammation. Nonetheless, these data differ between studies due to the probiotic bacterial strains used and the poor knowledge of their mechanisms of action.DesignBy mass-spectrometry, we identified and quantified free long chain fatty acids (LCFAs) in probiotics and assessed the effect of one of them in mouse colitis.ResultsAmong all the LCFAs quantified by mass spectrometry in Escherichia coli Nissle 1917 (EcN), a probiotic used for the treatment of multiple intestinal disorders, the concentration of 3-hydroxyoctadecaenoic acid (C18-3OH) was increased in EcN compared with other E. coli strains tested. Oral administration of C18-3OH decreased colitis induced by dextran sulfate sodium in mice. To determine whether other bacteria composing the microbiota are able to produce C18-3OH, we targeted the gut microbiota of mice with prebiotic fructooligosaccharides (FOS). The anti-inflammatory properties of FOS were associated with an increase in colonic C18-3OH concentration. Microbiota analyses revealed that the concentration of C18-3OH was correlated with an increase in the abundance in Allobaculum, Holdemanella and Parabacteroides. In culture, Holdemanella biformis produced high concentration of C18-3OH. Finally, using TR-FRET binding assay and gene expression analysis, we demonstrated that the C18-3OH is an agonist of peroxisome proliferator activated receptor gamma.ConclusionThe production of C18-3OH by bacteria could be one of the mechanisms implicated in the anti-inflammatory properties of probiotics. The production of LCFA-3OH by bacteria could be implicated in the microbiota/host interactions.
- Subjects :
- Cell Membrane Permeability
Chemokine CXCL1
medicine.medical_treatment
Interleukin-1beta
Gene Expression
Oligosaccharides
Peroxisome proliferator-activated receptor
Pancreatitis-Associated Proteins
Gut flora
medicine.disease_cause
Mass Spectrometry
law.invention
Mice
Peyer's Patches
Probiotic
0302 clinical medicine
law
Intestinal Mucosa
chemistry.chemical_classification
0303 health sciences
biology
Dextran Sulfate
Gastroenterology
Colitis
Lipids
Enteric bacterial microflora
Experimental colitis
3. Good health
enteric bacterial microflora
030211 gastroenterology & hepatology
lipids (amino acids, peptides, and proteins)
Long chain fatty acid
experimental colitis
Firmicutes
Permeability
Microbiology
lipids
03 medical and health sciences
Stearates
Escherichia coli
medicine
[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Animals
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
030304 developmental biology
Bacteroidetes
Probiotics
Prebiotic
Epithelial Cells
medicine.disease
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Gastrointestinal Microbiome
PPAR gamma
Prebiotics
chemistry
probiotics
Zonula Occludens-1 Protein
Caco-2 Cells
Bacteria
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Subjects
Details
- Language :
- English
- ISSN :
- 00175749
- Database :
- OpenAIRE
- Journal :
- Gut, Gut, BMJ Publishing Group, In press, 70 (6), pp.1088-1097. ⟨10.1136/gutjnl-2020-321173⟩, Gut, Vol. 70, p. 1088-1097 (2021)
- Accession number :
- edsair.doi.dedup.....01c3f3ba22b549f30d4760748ccf763b