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Metabolites of bovine-associated non-aureus staphylococci influence expression of Staphylococcus aureus agr-related genes in vitro
- Source :
- VETERINARY RESEARCH, Veterinary Research, Veterinary Research, Vol 52, Iss 1, Pp 1-14 (2021)
- Publication Year :
- 2021
-
Abstract
- Communications via quorum sensing (QS) between non-aureus staphylococci (NAS) and Staphylococcus (S.) aureus in the bovine mammary gland remains largely unexplored. We determined whether 34 S. chromogenes, 11 S. epidermidis, and 14 S. simulans isolates originating from bovine milk samples and teat apices were able to regulate the QS of S. aureus, and if so, how in vitro growth inhibition of S. aureus by NAS, or NAS metabolites, or NAS cells themselves play a role in this process. In co-culture with S. aureus we observed that these 3 NAS species in general downregulated the expression of rnaIII, the effector molecule of the QS system, but this effect was more pronounced in S. chromogenes and S. simulans isolates than in S. epidermidis isolates. In vitro growth inhibition of S. aureus by NAS resulted in a small underestimation of the downregulating effect of NAS on rnaIII expression of S. aureus. Additionally, the culture supernatant of these NAS isolates and supernatant treated with proteinase K expressed greater regulatory activity over S. aureus virulence genes rnaIII, hla, and spa than washed NAS cells suspended in sterile water. These microbial interactions may influence S. aureus virulence and pathogenesis within the host. Isolation and identification of NAS metabolites affecting the QS system of S. aureus might help to develop alternative strategies for treatment and control of S. aureus mastitis.
- Subjects :
- Staphylococcus aureus
SOMATIC-CELL COUNT
RNAIII
Veterinary medicine
Staphylococcus
agr
INHIBITION
Virulence
Down-Regulation
Mastitis
Biology
medicine.disease_cause
Microbiology
COLONIZATION
MASTITIS PATHOGENS
Mammary Glands, Animal
Bacterial Proteins
SF600-1100
INFECTION
medicine
Animals
Veterinary Sciences
sensing
DAIRY-COWS
General Veterinary
Effector
COAGULASE-NEGATIVE STAPHYLOCOCCI
STRAINS
BIOFILM FORMATION
Biofilm
Coagulase-negative staphylococci
Quorum Sensing
Gene Expression Regulation, Bacterial
In vitro
Quorum sensing
MILK-YIELD
Milk
Trans-Activators
INTRAMAMMARY
Quorum
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 09284249 and 12979716
- Database :
- OpenAIRE
- Journal :
- VETERINARY RESEARCH, Veterinary Research, Veterinary Research, Vol 52, Iss 1, Pp 1-14 (2021)
- Accession number :
- edsair.doi.dedup.....01c8bf65cc27f0693bffd79b379b1f3c