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Chondrogenic Priming at Reduced Cell Density Enhances Cartilage Adhesion of Equine Allogeneic MSCs - a Loading Sensitive Phenomenon in an Organ Culture Study with 180 Explants
- Source :
- Cellular Physiology and Biochemistry, 37, Cellular Physiology and Biochemistry, Vol 37, Iss 2, Pp 651-665 (2015), CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
- Publication Year :
- 2015
- Publisher :
- S. Karger AG, 2015.
-
Abstract
- Background: Clinical results of regenerative treatments for osteoarthritis are becoming increasingly significant. However, several questions remain unanswered concerning mesenchymal stem cell (MSC) adhesion and incorporation into cartilage. Methods: To this end, peripheral blood (PB) MSCs were chondrogenically induced and/or stimulated with pulsed electromagnetic fields (PEMFs) for a brief period of time just sufficient to prime differentiation. In an organ culture study, PKH26 labelled MSCs were added at two different cell densities (0.5 x106 vs 1.0 x106). In total, 180 explants of six horses (30 per horse) were divided into five groups: no lesion (i), lesion alone (ii), lesion with naïve MSCs (iii), lesion with chondrogenically-induced MSCs (iv) and lesion with chondrogenically-induced and PEMF-stimulated MSCs (v). Half of the explants were mechanically loaded and compared with the unloaded equivalents. Within each circumstance, six explants were histologically evaluated at different time points (day 1, 5 and 14). Results: COMP expression was selectively increased by chondrogenic induction (p = 0.0488). PEMF stimulation (1mT for 10 minutes) further augmented COL II expression over induced values (p = 0.0405). On the other hand, MSC markers remained constant over time after induction, indicating a largely predifferentiated state. In the unloaded group, MSCs adhered to the surface in 92.6% of the explants and penetrated into 40.7% of the lesions. On the other hand, physiological loading significantly reduced surface adherence (1.9%) and lesion filling (3.7%) in all the different conditions (p < 0.0001). Remarkably, homogenous cell distribution was characteristic for chondrogenic induced MSCs (+/- PEMFs), whereas clump formation occurred in 39% of uninduced MSC treated cartilage explants. Finally, unloaded explants seeded with a moderately low density of MSCs exhibited greater lesion filling (p = 0.0022) and surface adherence (p = 0.0161) than explants seeded with higher densities of MSCs. In all cases, the overall amount of lesion filling decreased from day 5 to 14 (p = 0.0156). Conclusion: The present study demonstrates that primed chondrogenic induction of MSCs at a lower cell density without loading results in significantly enhanced and homogenous MSC adhesion and incorporation into equine cartilage.<br />Cellular Physiology and Biochemistry, 37<br />ISSN:1015-8987<br />ISSN:1421-9778
- Subjects :
- Pathology
Physiology
MSCs
Cell Count
Osteoarthritis
Cartilage Oligomeric Matrix Protein
Horse
lcsh:Physiology
DOUBLE-BLIND
lcsh:QD415-436
Cells, Cultured
lcsh:QP1-981
biology
Cell Differentiation
DEFECTS
Chondrogenic
medicine.anatomical_structure
ANIMAL-MODELS
KNEE
medicine.symptom
Chondrogenesis
medicine.medical_specialty
Organ culture
MESENCHYMAL STEM-CELLS
lcsh:Biochemistry
Lesion
Electromagnetic Fields
Organ Culture Techniques
THICKNESS
Cell Adhesion
medicine
Animals
ELECTROMAGNETIC-FIELD THERAPY
Veterinary Sciences
Horses
Cartilage
Peripheral Blood
Cell adhesion
Collagen Type II
Cartilage oligomeric matrix protein
business.industry
Mesenchymal stem cell
Biology and Life Sciences
Mesenchymal Stem Cells
IN-VITRO
medicine.disease
Surgery
JOINT DISEASE
OSTEOARTHRITIS
biology.protein
business
Subjects
Details
- ISSN :
- 14219778 and 10158987
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Cellular Physiology and Biochemistry
- Accession number :
- edsair.doi.dedup.....01c948dd888ee11c7e1727afe61f7865