Back to Search
Start Over
Norepinephrine regulates arginine vasopressin secretion in hypothalamic paraventricular nucleus relating with pain modulation
- Source :
- Neuropeptides. 43(4)
- Publication Year :
- 2009
-
Abstract
- Our previous study has pointed that arginine vasopressin (AVP) and norepinephrine (NA) are two most important bioactive substances that play a role in hypothalamic paraventricular nucleus (PVN) regulating pain process. The communication was designed to investigate the interaction between AVP and NA in the rat PVN during the pain process. We used the potassium iontophoresis inducing tail-flick to test the pain threshold, PVN push-pull perfusion to collect the samples, high performance chromatography (HPLC) to determine the NA concentration and radioimmunoassay (RIA) to measure the AVP concentration. The results showed that (1) pain stimulation increased both NA and AVP concentrations in the PVN perfusion liquid; (2) PVN administration of l-glutamate sodium increased AVP, not NA concentration in the PVN perfusion liquid; (3) AVP or d(CH(2))(5)Tyr(Et)DAVP (AVP-receptor antagonist) neither changed pain threshold, nor influenced NA concentration in the PVN perfusion liquid; (4) Microinjection of NA into PVN could increase pain threshold in a dose-dependent manner, while PVN administration with phentolamine (alpha-receptor antagonist), not propranolol (beta-receptor antagonist) decreased pain threshold; (5) Administration of NA increased AVP concentration, while phentolamine, not propranolol decreased AVP concentration in the PVN perfusion liquid. These data suggested that it is through alpha-receptor rather than beta-receptor, NA induced PVN secretion of AVP that was delivered to the related brain regions to participate in pain modulation.
- Subjects :
- Male
Pain Threshold
endocrine system
Vasopressin
medicine.medical_specialty
Adrenergic Antagonists
Radioimmunoassay
Neuropeptide
Pain
Propranolol
Rats, Sprague-Dawley
Cellular and Molecular Neuroscience
Norepinephrine
Endocrinology
Phentolamine
Internal medicine
Threshold of pain
medicine
Animals
Pain Measurement
Endocrine and Autonomic Systems
Chemistry
digestive, oral, and skin physiology
General Medicine
Iontophoresis
Rats
Arginine Vasopressin
nervous system
Neurology
Vasopressin secretion
Hypothalamus
Catecholamine
Adrenergic alpha-Agonists
hormones, hormone substitutes, and hormone antagonists
medicine.drug
Paraventricular Hypothalamic Nucleus
Subjects
Details
- ISSN :
- 15322785
- Volume :
- 43
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Neuropeptides
- Accession number :
- edsair.doi.dedup.....022498c4d2ff4d7a977d11a07978d168