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Chronic Cigarette Smoke Causes Oxidative Damage and Apoptosis to Retinal Pigmented Epithelial Cells in Mice
- Source :
- PLoS ONE, Vol 3, Iss 9, p e3119 (2008), PLoS ONE
- Publication Year :
- 2008
- Publisher :
- Public Library of Science (PLoS), 2008.
-
Abstract
- The purpose of this study was to determine whether mice exposed to chronic cigarette smoke develop features of early age-related macular degeneration (AMD). Two month old C57Bl6 mice were exposed to either filtered air or cigarette smoke in a smoking chamber for 5 h/day, 5 days/week for 6 months. Eyes were fixed in 2.5% glutaraldehyde/2% paraformaldehyde and examined for ultrastructural changes by transmission electron microscopy. The contralateral eye was fixed in 2% paraformaldehyde and examined for oxidative injury to the retinal pigmented epithelium (RPE) by 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-OHdG) immunolabeling and apoptosis by TUNEL labeling. Mice exposed to cigarette smoke had immunolabeling for 8-OHdG in 85+/-3.7% of RPE cells counted compared to 9.5+/-3.9% in controls (p
- Subjects :
- Pathology/Histopathology
Pathology
medicine.medical_specialty
Time Factors
lcsh:Medicine
Apoptosis
Retinal Pigment Epithelium
Biology
medicine.disease_cause
Andrology
Macular Degeneration
Mice
03 medical and health sciences
Basal (phylogenetics)
chemistry.chemical_compound
0302 clinical medicine
In Situ Nick-End Labeling
medicine
Geriatrics/Geriatric Ophthalmology
Animals
Humans
lcsh:Science
Paraformaldehyde
030304 developmental biology
Smoke
0303 health sciences
Multidisciplinary
Retinal pigment epithelium
TUNEL assay
Smoking
lcsh:R
Epithelial Cells
Retinal
Ophthalmology/Macular Disorders
eye diseases
Mice, Inbred C57BL
Oxidative Stress
medicine.anatomical_structure
chemistry
030221 ophthalmology & optometry
Tobacco Smoke Pollution
lcsh:Q
Bruch Membrane
sense organs
Oxidative stress
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....02341b40728fd382471985894596f66a