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Regulatory T cells promote adipocyte beiging in subcutaneous adipose tissue
- Source :
- FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES. 34(7)
- Publication Year :
- 2019
-
Abstract
- Regulatory T cells (Tregs) play essential roles in obesity and diabetes. Here, we report a role of Tregs in enhancing β3-adrenergic receptor agonist CL316243 (CL)-stimulated thermogenic program in subcutaneous adipose tissue (SAT), but not in visceral fat. CL treatment for 7 days increased SAT adipocyte beiging and thermogenic gene expression in male or female mice. Adoptive transfer of Tregs enhanced this CL activity. Such Treg activity lost in male epididymal white adipose tissue (eWAT) and female gonadal gWAT. Adipocyte culture yielded the same conclusion. Tregs enhanced the expression of CL-induced thermogenic genes in SAT from male and female mice. This activity of Tregs reduced or disappeared in adipocytes from eWAT or gWAT. Both CL and Tregs induced much higher UCP-1 (uncoupling protein-1) expression in SAT from females than that from males. A mechanistic study demonstrated a role of Tregs in suppressing the expression of M1 macrophage markers (Tnfa, Il6, iNos, Ip10) and promoting the expression of M2 macrophage markers (Mrc1, Arg1, Il10) in bone-marrow-derived macrophages or in SAT from male or female mice. In female mice with pre-established obesity, Treg adoptive transfer reduced the gWAT weight in 2 weeks. Together with CL treatment, Treg adoptive transfer reduced the SAT weight and further improved CL-induced glucose metabolism and insulin sensitivity in female obese mice, but did not affect CL-induced body weight loss in male or female obese mice. This study revealed a predominant role of Tregs in female mice in promoting adipocyte beiging and thermogenesis in SAT, in part by slanting M2 macrophage polarization.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Regulatory T cell
Adipose Tissue, White
Macrophage polarization
Subcutaneous Fat
Mice, Obese
chemical and pharmacologic phenomena
White adipose tissue
Biology
Biochemistry
T-Lymphocytes, Regulatory
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Adipose Tissue, Brown
Internal medicine
Adipocyte
Genetics
medicine
Animals
Obesity
Molecular Biology
hemic and immune systems
Thermogenesis
M2 Macrophage
Thermogenin
Mice, Inbred C57BL
Interleukin 10
030104 developmental biology
medicine.anatomical_structure
Endocrinology
chemistry
Female
Insulin Resistance
Energy Metabolism
030217 neurology & neurosurgery
Biotechnology
Subjects
Details
- ISSN :
- 15306860
- Volume :
- 34
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES
- Accession number :
- edsair.doi.dedup.....025071726f2a24e8a693454b8964b05e