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Nanogels Derived from Fish Gelatin: Application to Drug Delivery System
- Source :
- Marine Drugs, Volume 17, Issue 4, Marine Drugs, Vol 17, Iss 4, p 246 (2019)
- Publication Year :
- 2019
-
Abstract
- The gelatin extracted from mammals of porcine and bovine has been prominently used in pharmaceutical, medical, and cosmetic products. However, there have been some concerns for their usage due to religious, social and cultural objections, and animal-to-human infectious disease. Recently, gelatin from marine by-products has received growing attention as an alternative to mammalian gelatin. In this study, we demonstrate the formation of nanogels (NGs) using fish gelatin methacryloyl (GelMA) and their application possibility to the drug delivery system. The fabrication of fish GelMA NGs is carried out by crosslinking through the photopolymerization of the methacryloyl substituent present in the nanoemulsion droplets, followed by purification and redispersion. There were different characteristics depending on the aqueous phase in the emulsion and the type of solvent used in redispersion. The PBS-NGs/D.W., which was prepared using PBS for the aqueous phase and D.W. for the final dispersion solution, had a desirable particle size (&lt<br />200 nm), low PdI (0.16), and high drug loading efficiency (77%). Spherical NGs particles were observed without aggregation in TEM images. In vitro release tests of doxorubicin (DOX)-GelMA NGs showed the pH-dependent release behavior of DOX. Also, the MTT experiments demonstrated that DOX-GelMA NGs effectively inhibited cell growth, while only GelMA NGs exhibit higher percentages of cell viability. Therefore, the results suggest that fish GelMA NGs have a potential for nano-carrier as fine individual particles without the aggregation and cytotoxicity to deliver small-molecule drugs.
- Subjects :
- food.ingredient
Cell Survival
Pharmaceutical Science
02 engineering and technology
Gelatin
Article
Cell Line
03 medical and health sciences
Mice
food
Drug Delivery Systems
fish gelatin
marine by-products
Drug Discovery
Animals
drug delivery system
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
lcsh:QH301-705.5
gelatin methacryloyl
030304 developmental biology
0303 health sciences
Chromatography
Aqueous two-phase system
Fishes
Hydrogels
021001 nanoscience & nanotechnology
Solvent
Photopolymer
lcsh:Biology (General)
Doxorubicin
Drug delivery
Emulsion
nanogel
NIH 3T3 Cells
Nanoparticles
Particle size
0210 nano-technology
Nanogel
Subjects
Details
- ISSN :
- 16603397
- Volume :
- 17
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Marine drugs
- Accession number :
- edsair.doi.dedup.....026a87c50bb13c31aa627f38958ef661