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Datasets of whole cell and mitochondrial oxysterols derived from THP-1, SH-SY5Y and human peripheral blood mononuclear cells using targeted metabolomics
- Source :
- Data in Brief, Vol 33, Iss, Pp 106382-(2020), Data in Brief
- Publication Year :
- 2020
-
Abstract
- The raw datasets of oxysterol quantifications from whole cell and mitochondrial fractions of THP-1 monocytes and macrophages, neuronal-like SH-SH5Y cells and human peripheral blood mononuclear cells are presented. Oxysterols were quantified using a new liquid chromatography-mass spectrometry (LC-MS) and multiple reaction monitoring analysis published in the article "A quantitative LC-MS/MS method for analysis of mitochondrial-specific oxysterol metabolism" in Redox Biology [1]. This method showed improved extraction efficiency and recovery of mono and dihydroxycholesterols from cellular matrix. The datasets derived from the three cell lines are included in the appendix. These datasets provide new information about the oxysterol distribution in THP-1 monocytes and macrophages, SH-SY5Y cells and peripheral blood mononuclear cells. These datasets can be used as a guide for oxysterol distribution in the three cell lines for future studies, and can used for future method optimization, and for comparison of oxysterol recovery with other analytical techniques.
- Subjects :
- SH-SY5Y
Oxysterol
Mitochondrion
lcsh:Computer applications to medicine. Medical informatics
Peripheral blood mononuclear cell
Extracellular matrix
03 medical and health sciences
0302 clinical medicine
Liquid chromatography–mass spectrometry
polycyclic compounds
THP1 cell line
Liquid chromatography-mass spectrometry
lcsh:Science (General)
030304 developmental biology
Data Article
0303 health sciences
Multidisciplinary
Chemistry
Cell biology
Mitochondria
Cholesterol
Metabolism
Cell culture
lcsh:R858-859.7
lipids (amino acids, peptides, and proteins)
030217 neurology & neurosurgery
lcsh:Q1-390
Subjects
Details
- Language :
- English
- ISSN :
- 23523409
- Database :
- OpenAIRE
- Journal :
- Data in Brief, Vol 33, Iss, Pp 106382-(2020), Data in Brief
- Accession number :
- edsair.doi.dedup.....02c64895f8ca07abf0b1a614bcbf10ce