Back to Search
Start Over
Chitin binding protein from the kuruma shrimp Marsupenaeus japonicus facilitates the clearance of Vibrio anguillarum
- Source :
- Developmental and comparative immunology. 117
- Publication Year :
- 2020
-
Abstract
- Peritrophic membrane (PM) refers to a vital physical barrier enabling shrimp to resist pathogen invasion. It primarily consists of chitin and proteins, mostly chitin-binding protein (CBP). CBPs have been identified from microorganisms to higher organisms. In the present study, a CBP, designated MjCBP, was reported from Marsupenaeus japonicus. The open reading frame of MjCBP was 1854 bp, encoding a protein with 618 amino acids (MH544098). To be specific, the theoretical pI and molecular mass of mature MjCBP reached 5.43 and 66064.00 Da, respectively. MjCBP consisted of seven type Ⅱ chitin-binding domains (ChtB D2), which was up-regulated after being challenged with Vibrio anguillarum and then agglutinating several bacteria. In addition, MjCBP and the first chitin-binding domain (CBD1) could bind to several Gram-positive and Gram-negative bacteria via the binding process to lipopolysaccharides and peptidoglycans, whereas CBD1 was not capable of agglutinating bacteria. Moreover, the anterior and posterior segments of CBD1 were synthesized in vitro, and the posterior segment could bind to lipopolysaccharides. However, both segments fail to agglutinate bacteria. Furthermore, MjCBP and CBD1 facilitated the clearance of V. anguillarum in vivo, and the silencing of MjCBP via RNA interference reduced the ability of bacterial clearance. As revealed from the mentioned results, MjCBP acts as an opsonin or pattern recognition receptor to achieve antibacterial immune response in shrimp.
- Subjects :
- 0301 basic medicine
Vibrio anguillarum
Hemocytes
Immunology
Chitin
01 natural sciences
Microbiology
Arthropod Proteins
03 medical and health sciences
chemistry.chemical_compound
Penaeidae
Chitin binding
Animals
Amino Acid Sequence
0101 mathematics
Opsonin
Vibrio
Innate immune system
biology
Sequence Homology, Amino Acid
Gene Expression Profiling
biology.organism_classification
Immunity, Innate
Shrimp
010101 applied mathematics
Open reading frame
030104 developmental biology
chemistry
Host-Pathogen Interactions
RNA Interference
Carrier Proteins
Bacteria
Developmental Biology
Protein Binding
Subjects
Details
- ISSN :
- 18790089
- Volume :
- 117
- Database :
- OpenAIRE
- Journal :
- Developmental and comparative immunology
- Accession number :
- edsair.doi.dedup.....02d5ace303c92f839e7dbb96c466448d