Back to Search
Start Over
Alterations in Intestinal Microbiota of Children With Celiac Disease at the Time of Diagnosis and on a Gluten-free Diet
- Source :
- Gastroenterology
- Publication Year :
- 2020
- Publisher :
- W.B. Saunders, 2020.
-
Abstract
- Background And Aims It is not clear whether alterations in the intestinal microbiota of children with celiac disease (CD) cause the disease or are a result of disease and/or its treatment with a gluten-free diet (GFD). Methods We obtained 167 fecal samples from 141 children (20 with new-onset CD, 45 treated with a GFD, 57 healthy children, and 19 unaffected siblings of children with CD) in Glasgow, Scotland. Samples were analyzed by 16S ribosomal RNA sequencing, and diet-related metabolites were measured by gas chromatography. We obtained fecal samples from 13 children with new-onset CD after 6 and 12 months on a GFD. Relationships between microbiota with diet composition, gastrointestinal function, and biomarkers of GFD compliance were explored. Results Microbiota α diversity did not differ among groups. Microbial dysbiosis was not observed in children with new-onset CD. In contrast, 2.8% (Bray-Curtis dissimilarity index, P = .025) and 2.5% (UniFrac distances, P = .027) of the variation in microbiota composition could be explained by the GFD. Between 3% and 5% of all taxa differed among all group comparisons. Eleven distinctive operational taxonomic units composed a microbe signature specific to CD with high diagnostic probability. Most operational taxonomic units that differed between patients on a GFD with new-onset CD vs healthy children were associated with nutrient and food group intake (from 75% to 94%) and with biomarkers of gluten ingestion. Fecal levels of butyrate and ammonia decreased during the GFD. Conclusions Although several alterations in the intestinal microbiota of children with established CD appear to be effects of a GFD, specific bacteria were found to be distinct biomarkers of CD. Studies are needed to determine whether these bacteria contribute to pathogenesis of CD.<br />Graphical abstract
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
OTU
UCD, untreated celiac disease
Gastroenterology
Pathogenesis
Food group
03 medical and health sciences
0302 clinical medicine
Internal medicine
medicine
GFD, gluten-free diet
OTU, operational taxonomic unit
Ingestion
HC, healthy control subjects
Microbiome
GIP, gluten immunogenic peptide
Feces
Original Research
chemistry.chemical_classification
Pediatric
Hepatology
business.industry
Short-chain Fatty Acids
GI, gastrointestinal
Gluten
030104 developmental biology
chemistry
CD, celiac disease
SCFA, short-chain fatty acid
TCD, treated celiac disease
030211 gastroenterology & hepatology
Gluten free
Clinical—Alimentary Tract
Gastrointestinal function
business
BCFA, branch-chain fatty acid
Subjects
Details
- Language :
- English
- ISSN :
- 15280012 and 00165085
- Volume :
- 159
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Gastroenterology
- Accession number :
- edsair.doi.dedup.....02e2093e6f80a277f8835766d511f462