Back to Search
Start Over
Endogenous Glutamate Increases Extracellular Concentrations of Dopamine, GABA, and Taurine Through NMDA and AMPA/Kainate Receptors in Striatum of the Freely Moving Rat: A Microdialysis Study
- Source :
- ResearcherID
- Publication Year :
- 1997
- Publisher :
- Wiley, 1997.
-
Abstract
- Interactions between glutamate (Glu), dopamine (DA), GABA, and taurine (Tau) were investigated in striatum of the freely moving rat by using microdialysis. Intrastriatal infusions of the selective Glu uptake inhibitor l-trans-pyrrolidine-3,4-dicarboxylic acid (PDC) were used to increase the endogenous extracellular [Glu]. Correlations between extracellular [Glu] and extracellular [DA], [GABA], and [Tau], and the effects of a selective blockade of ionotropic Glu receptors, were studied. PDC (1, 2, and 4 mM) produced a dose-related increase in extracellular [Glu]. At the highest dose of PDC, [Glu] increased from 1.55 ± 0.35 to 6.11 ± 0.88 µM. PDC also increased extracellular [DA], [GABA], and [Tau]. The increasing [Glu] was correlated significantly with increasing [DA], [GABA], and [Tau]. PDC also decreased extracellular concentrations of DA metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and 4-hydroxy-3-methoxyphenylacetic acid (HVA). Perfusion with the NMDA-receptor antagonist 3-[(R)-2-carboxypiperazin-4-yl]-propyl-1-phosphonic acid (1 mM) or the AMPA/kainate-receptor antagonist 6,7-dinitroquinoxaline-2,3-dione (DNQX) (1 mM) attenuated the increases produced by PDC (4 mM) on [DA], [GABA], and [Tau], and decreases in [DOPAC] and [HVA]. DNQX also attenuated the increases in [Glu] induced by PDC. These data show that endogenous Glu plays a role in modulating the extracellular concentrations of DA, GABA, and Tau in striatum of the freely moving rat.
- Subjects :
- Male
medicine.medical_specialty
Taurine
Microdialysis
Pyrrolidines
Dopamine
Glutamic Acid
AMPA receptor
Receptors, N-Methyl-D-Aspartate
Biochemistry
Cellular and Molecular Neuroscience
chemistry.chemical_compound
Internal medicine
DNQX
Extracellular
medicine
Animals
Dicarboxylic Acids
Receptors, AMPA
Rats, Wistar
gamma-Aminobutyric Acid
Osmolar Concentration
Glutamate receptor
Corpus Striatum
Rats
Endocrinology
Receptors, Glutamate
nervous system
chemistry
NMDA receptor
Extracellular Space
Ionotropic effect
Subjects
Details
- ISSN :
- 00223042
- Volume :
- 69
- Database :
- OpenAIRE
- Journal :
- Journal of Neurochemistry
- Accession number :
- edsair.doi.dedup.....0320d411e8f8d25ba856becad61b9d08