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Regulation of antigen-specific immunoglobulin G subclasses in response to conserved and polymorphic Plasmodium falciparum antigens in an in vitro model
- Source :
- Infection and Immunity, Infection and Immunity, American Society for Microbiology, 2002, 70 (6), pp.2820-7. ⟨10.1128/IAI.70.6.2820-2827.2002⟩, Infection and Immunity, 2002, 70 (6), pp.2820-7. ⟨10.1128/IAI.70.6.2820-2827.2002⟩
- Publication Year :
- 2002
-
Abstract
- Cytophilic antibodies (Abs) play a critical role in protection againstPlasmodium falciparumblood stages, yet little is known about the parameters regulating production of these Abs. We used an in vitro culture system to study the subclass distribution of antigen (Ag)-specific immunoglobulin G (IgG) produced by peripheral blood mononuclear cells (PBMCs) from individuals exposed toP. falciparumor unexposed individuals. PBMCs, cultivated with or without cytokines and exogenous CD40/CD40L signals, were stimulated with a crude parasite extract, recombinant vaccine candidates derived from conserved Ags (19-kDa C terminus of merozoite surface protein 1 [MSP119], R23, and PfEB200), or recombinant Ags derived from the polymorphic Ags MSP1 block 2 and MSP2. NoP. falciparum-specific Ab production was detected in PBMCs from unexposed individuals. PBMCs from donors exposed frequently toP. falciparuminfections produced multiple IgG subclasses when they were stimulated with the parasite extract but usually only one IgG subclass when they were stimulated with a recombinant Ag. Optimal Ab production required addition of interleukin-2 (IL-2) and IL-10 for all antigenic preparations. The IgG subclass distribution was both donor and Ag dependent and was only minimally influenced by the exogenous cytokine environment. In vitro IgG production and subclass distribution correlated with plasma Abs to some Ags (MSP119, R23, and MSP2) but not others (PfEB200 and the three MSP1 block 2-derived Ags). Data presented here suggest that intrinsic properties of the protein Ag itself play a major role in determining the subclass of the Ab response, which has important implications for rational design of vaccine delivery.
- Subjects :
- Male
Endemic Diseases
Protozoan Proteins
Antibodies, Protozoan
Subclass
Immunoglobulin G
law.invention
0302 clinical medicine
law
MESH: Animals
Malaria, Falciparum
MESH: Protozoan Proteins
MESH: Plasmodium falciparum
Cells, Cultured
Conserved Sequence
Merozoite Surface Protein 1
MESH: Merozoite Surface Protein 1
MESH: Immunoglobulin G
0303 health sciences
MESH: Cytokines
MESH: Conserved Sequence
MESH: Malaria, Falciparum
Isotype
Senegal
3. Good health
Immunoglobulin Isotypes
MESH: Leukocytes, Mononuclear
Infectious Diseases
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
MESH: Endemic Diseases
Recombinant DNA
Cytokines
Female
Antibody
MESH: Cells, Cultured
Adult
Recombinant Fusion Proteins
Immunology
Plasmodium falciparum
Antigens, Protozoan
Biology
Microbiology
MESH: Models, Immunological
03 medical and health sciences
Antigen
MESH: Senegal
MESH: Polymorphism, Genetic
parasitic diseases
MESH: Recombinant Fusion Proteins
MESH: Antibodies, Protozoan
Animals
Humans
030304 developmental biology
MESH: Humans
Polymorphism, Genetic
Models, Immunological
MESH: Adult
biology.organism_classification
Virology
MESH: Male
MESH: Immunoglobulin Isotypes
Humoral immunity
biology.protein
Leukocytes, Mononuclear
Parasitology
Fungal and Parasitic Infections
MESH: Female
030215 immunology
MESH: Antigens, Protozoan
Subjects
Details
- ISSN :
- 00199567 and 10985522
- Volume :
- 70
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Infection and immunity
- Accession number :
- edsair.doi.dedup.....034cafea201e50b624f0c19c4b9c1434