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Transcriptome of porcine alveolar macrophages activated by interferon-gamma and lipopolysaccharide
- Source :
- Biochemical and Biophysical Research Communications. 503:2666-2672
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- The molecular repertoire of porcine alveolar macrophages (PAMs) is greatly affected by the microenvironment they are exposed to, and specifically by inflammatory cytokines, such as interferon gamma (IFN-γ) released by activated lymphocytes, and microbial products, such as lipopolysaccharide (LPS). In our previous study, we found that IFN-γ- and LPS-activated PAMs (M1) could inhibit porcine reproductive and respiratory syndrome virus (PRRSV) replication. In this study, comprehensive analysis of the expression profiles of the genes associated with the polarization of M0-type PAMs (resting) toward M1 phenotypes (activated by IFN-γ and LPS) led to the following main results: 1) 1551 and 1823 genes were upregulated or downregulated in M1-type PAMs, respectively, compared with M0-type PAMs; 2) Among these, genes encoding ASS1 and CRTAM were the most upregulated and downregulated, respectively; 3) Genes involved in cytokine-cytokine receptor interaction and the JAK/STAT signaling pathway were significantly upregulated, suggesting their critical role in cellular activation; and 4) Genes involved in antigen proteolysis and presentation (immunoproteasome subunits), and inhibition of virus replication (host restriction factors) were significantly upregulated, emphasizing the critical role of these cytokines in immunity. Thus, our results provide important information for future studies on the role of PAM polarization in modulation of infection.
- Subjects :
- Lipopolysaccharides
0301 basic medicine
Lipopolysaccharide
Swine
Primary Cell Culture
Biophysics
Immunoglobulins
Argininosuccinate Synthase
Biology
Virus Replication
Biochemistry
Proinflammatory cytokine
Transcriptome
Interferon-gamma
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Downregulation and upregulation
Macrophages, Alveolar
medicine
Animals
Cell Lineage
Porcine respiratory and reproductive syndrome virus
Interferon gamma
Receptors, Cytokine
Molecular Biology
Cell Differentiation
Molecular Sequence Annotation
Janus Kinase 1
Cell Biology
Porcine reproductive and respiratory syndrome virus
biology.organism_classification
Phenotype
Cell biology
STAT Transcription Factors
Gene Ontology
030104 developmental biology
chemistry
Viral replication
030215 immunology
medicine.drug
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 503
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....03822b16cca6154344b7fbc82a0215ee