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1H-NMR metabolomics response to a realistic diet contamination with the mycotoxin deoxynivalenol: Effect of probiotics supplementation
- Source :
- Food and Chemical Toxicology, Food and Chemical Toxicology, Elsevier, 2020, in press, ⟨10.1016/j.fct.2020.111222⟩
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- International audience; Low-level contamination of food and feed by deoxynivalenol (DON) is unavoidable. We investigated the effects of subclinical treatment with DON, and supplementation with probiotic yeast Saccharomyces cerevisiae boulardii I1079 as a preventive strategy in piglets. Thirty-six animals were randomly assigned to either a control diet, a diet contaminated with DON (3 mg/kg), a diet supplemented with yeast (4 × 109 CFU/kg), or a DON-contaminated diet supplemented with yeast, for four weeks. Plasma and tissue samples were collected for biochemical analysis, 1H-NMR untargeted metabolomics, and histology. DON induced no significant modifications in biochemical parameters. However, lesion scores were higher and metabolomics highlighted alterations of amino acid and 2-oxocarboxylic acid metabolism. Administering yeast affected aminoacyl-tRNA synthesis and amino acid and glycerophospholipid metabolism. Yeast supplementation of piglets exposed to DON prevented histological alterations, and partial least square discriminant analysis emphasised similarity between the metabolic profiles of their plasma and that of the control group. The effect on liver metabolome remained marginal, indicating that the toxicity of the mycotoxin was not eliminated. These findings show that the 1H-NMR metabolomics profile is a reliable biomarker to assess subclinical exposure to DON, and that supplementation with S. cerevisiae boulardii increases the resilience of piglets to this mycotoxin.
- Subjects :
- pig
Biology
Toxicology
law.invention
histology
03 medical and health sciences
chemistry.chemical_compound
Probiotic
0404 agricultural biotechnology
Metabolomics
law
mycotoxins
Metabolome
Food science
Mycotoxin
intestine
030304 developmental biology
Subclinical infection
2. Zero hunger
0303 health sciences
Saccharomyces cerevisiae boulardii
04 agricultural and veterinary sciences
General Medicine
Metabolism
040401 food science
metabolomics
Yeast
3. Good health
chemistry
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Toxicity
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 02786915
- Database :
- OpenAIRE
- Journal :
- Food and Chemical Toxicology, Food and Chemical Toxicology, Elsevier, 2020, in press, ⟨10.1016/j.fct.2020.111222⟩
- Accession number :
- edsair.doi.dedup.....03c9008a21546ffaa4ab4aa13f4d1819