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Purification, characterization and immunomodulating activity of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.)
- Source :
- International Immunopharmacology. 17:858-866
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- A water-soluble polysaccharide (JS-MP-1) was isolated and purified from the Korean mulberry fruits Oddi (Morus alba L.) by crushing the fresh fruits then performing ethanol precipitation and DEAE-cellulose ion exchange chromatography. The neutral monosaccharide composition of the purified JS-MP-1 was determined to be composed mainly of galactose (37.6%, in mole percent), arabinose (36.3%), and rhamnose (18.4%), while other major sugars such as glucose, xylose, mannose, and fucose were present as minor components. HPLC analysis revealed that JS-MP-1 contains both galacturonic acid (GalA) and glucuronic acid (GlcA) at approximately 4:1 in mole percent. Monosaccharide composition, Fourier-transform infrared (FTIR) analysis, biochemical analysis, and elemental analysis suggested that JS-MP-1 is an acidic heteropolysaccharide, most likely a rhamnoarabinogalacturonan type plant pectic polysaccharide, with an apparent molecular mass of 1600 kDa containing no, or if any, negligible level of sulfate esters and proteins. Enzyme-Linked Immunosorbent Assay and RT-PCR analysis demonstrated that JS-MP-1 significantly stimulates murine RAW264.7 macrophage cells to release chemokines (RANTES and MIP-1α) and proinflammatory cytokines (TNF-α and IL-6) and to induce the expression of iNOS and COX-2, which are responsible for the production of NO and prostaglandin PGE2, respectively. These results suggest that the mulberry fruit-derived polysaccharide JS-MP-1 can act as a potent immunomodulator, and these observations may support the applicability of this polysaccharide as an immunotherapeutic adjuvant or the water extracts of the mulberry fruit as a beneficial health food.
- Subjects :
- Arabinose
Rhamnose
Immunology
Nitric Oxide Synthase Type II
Mannose
Xylose
Polysaccharide
Fucose
Cell Line
Mice
chemistry.chemical_compound
Polysaccharides
Animals
Immunologic Factors
Immunology and Allergy
RNA, Messenger
Pharmacology
chemistry.chemical_classification
Monosaccharides
Glucuronic acid
Uronic Acids
chemistry
Biochemistry
Cyclooxygenase 2
Fruit
Galactose
Cytokines
Morus
Subjects
Details
- ISSN :
- 15675769
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- International Immunopharmacology
- Accession number :
- edsair.doi.dedup.....03e86633b43b42fff3c502103de9b582
- Full Text :
- https://doi.org/10.1016/j.intimp.2013.09.019