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Global DNA Methylation Changes in Nile Tilapia Gonads during High Temperature-Induced Masculinization
- Source :
- PLoS ONE, Vol 11, Iss 8, p e0158483 (2016), PLoS ONE
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- In some fish species, high or low temperature can switch the sex determination mechanisms and induce fish sex reversal when the gonads are undifferentiated. During this high or low temperature-induced sex reversal, the expressions of many genes are altered. However, genome-wide DNA methylation changes in fish gonads after high or low temperature treatment are unclear. Herein, we compared the global DNA methylation changes in the gonads from control females (CF), control males (CM), high temperature-treated females (TF), and high temperature-induced males (IM) from the F8 family of Nile tilapia (Oreochromis niloticus) using methylated DNA immunoprecipitation sequencing. The DNA methylation level in CF was higher than that in CM for various chromosomes. Both females and males showed an increase in methylation levels on various chromosomes after high-temperature induction. We identified 64,438 (CF/CM), 63,437 (TF/IM), 98,675 (TF/CF), 235,270 (IM/CM) and 119,958 (IM/CF) differentially methylated regions (DMRs) in Nile tilapia gonads, representing approximately 0.70% (CF/CM), 0.69% (TF/IM), 1.07% (TF/CF), 2.56% (IM/CM), and 1.30% (IM/CF)of the length of the genome. A total of 89 and 65 genes that exhibited DMRs in their gene bodies and promoters were mapped to the Nile tilapia genome. Furthermore, more than half of the genes with DMRs in the gene body in CF/CM were also included in the IM/CM, TF/CF, TF/IM, and IM/CF groups. Additionally, many important pathways, including neuroactive ligand-receptor interaction, extracellular matrix-receptor interaction, and biosynthesis of unsaturated fatty acids were identified. This study provided an important foundation to investigate the molecular mechanism of high temperature-induced sex reversal in fish species.
- Subjects :
- 0301 basic medicine
Male
Hot Temperature
lcsh:Medicine
Artificial Gene Amplification and Extension
Biochemistry
Polymerase Chain Reaction
Nile tilapia
0302 clinical medicine
Medicine and Health Sciences
Gene Regulatory Networks
lcsh:Science
Multidisciplinary
DNA methylation
biology
Fishes
Methylation
Cichlids
Sex reversal
Chromatin
Nucleic acids
Ovaries
Oreochromis
Vertebrates
Female
Epigenetics
Anatomy
DNA modification
Genital Anatomy
Chromatin modification
Research Article
Chromosome biology
Cell biology
Histology
Research and Analysis Methods
Chromosomes
03 medical and health sciences
Genetics
Animals
Methylated DNA immunoprecipitation
Gonads
Molecular Biology Techniques
Molecular Biology
Biology and life sciences
lcsh:R
Reproductive System
Organisms
Promoter
Sequence Analysis, DNA
DNA
Sex Determination Processes
biology.organism_classification
Molecular biology
030104 developmental biology
Differentially methylated regions
Gene Expression Regulation
lcsh:Q
Gene expression
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....0432c525dac300711176bdfdf4b16e33