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Circulating miRNA measurements are reflective of cholesterol-based changes in rainbow trout (Oncorhynchus mykiss)
- Source :
- Plos One 11 (13), e0206727. (2018), PLoS ONE, Vol 13, Iss 11, p e0206727 (2018), PLoS ONE, PLoS ONE, Public Library of Science, 2018, 13 (11), pp.e0206727. ⟨10.1371/journal.pone.0206727⟩
- Publication Year :
- 2018
-
Abstract
- International audience; MicroRNAs (miRNAs) are a class of small non-coding RNAs which are known to posttranscriptionally regulate the expression of most genes in both animals and plants. Meanwhile, studies have shown that numbers of miRNAs are present in body fluids including the plasma. Despite the mode of action of these circulating miRNAs still remains unknown, they have been found to be promising biomarkers for disease diagnosis, prognosis and response to treatment. In order to evaluate the potential of miRNAs as non-invasive biomarkers in aquaculture, a time-course experiment was implemented to investigate the postprandial regulation of miRNAs levels in liver and plasma as well as the hepatic expression of genes involved in cholesterol metabolism. We showed that miR-1, miR-33a, miR-122, miR-128 and miR-223 were expressed in the liver of rainbow trout and present at detectable level in the plasma. We also demonstrated that hepatic expression of miR-1, miR-122 and miR-128 were regulated by feed intake and reached their highest levels 12 hours after the meal. Interestingly, we observed that circulating levels of miR-128 and miR-223 are subjected to postprandial regulations similar to that observed in their hepatic counterparts. Statistical correlations were observed between liver and plasma for miR-128 and miR-223 and between hepatic and circulating miR-122, miR-128 and miR-223 and expression of genes related to cholesterol synthesis and efflux or glucose phosphorylation. These results demonstrated that circulating miR-122, miR-128 and miR-223 are potential biomarkers of cholesterol metabolism in rainbow trout.
- Subjects :
- 0301 basic medicine
Circulating mirnas
Blood Glucose
Physiology
lcsh:Medicine
Biochemistry
chemistry.chemical_compound
Medicine and Health Sciences
lcsh:Science
métabolisme du cholestérol
ComputingMilieux_MISCELLANEOUS
Animal biology
trout
oncorhynchus mykiss
Multidisciplinary
[SDV.BA]Life Sciences [q-bio]/Animal biology
Eukaryota
cholestérol
Postprandial Period
Lipids
rainbow trout
Body Fluids
microRNAs
Nucleic acids
Postprandial
Cholesterol
Blood
Liver
Osteichthyes
Vertebrates
micro arn
Efflux
Anatomy
Research Article
expression des gènes
medicine.medical_specialty
Fish Biology
Biology
Blood Plasma
03 medical and health sciences
rainbow
Internal medicine
microRNA
Biologie animale
medicine
Genetics
Fish Physiology
Animals
Animal Physiology
14. Life underwater
Non-coding RNA
Gene
Triglycerides
Natural antisense transcripts
Biology and life sciences
Gene Expression Profiling
Lipogenesis
lcsh:R
Organisms
Lipid Metabolism
Vertebrate Physiology
Gene regulation
Gene expression profiling
030104 developmental biology
Endocrinology
Fish
chemistry
RNA
Rainbow trout
lcsh:Q
Gene expression
Zoology
Biomarkers
truite arc en ciel
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- Plos One 11 (13), e0206727. (2018), PLoS ONE, Vol 13, Iss 11, p e0206727 (2018), PLoS ONE, PLoS ONE, Public Library of Science, 2018, 13 (11), pp.e0206727. ⟨10.1371/journal.pone.0206727⟩
- Accession number :
- edsair.doi.dedup.....045da50f2476f9017da49cd28131f668