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Evidence for sub-haplogroup h5 of mitochondrial DNA as a risk factor for late onset Alzheimer's disease
- Source :
- PLoS ONE, PLoS ONE, Vol 5, Iss 8, p e12037 (2010)
- Publication Year :
- 2010
-
Abstract
- BackgroundAlzheimer's Disease (AD) is the most common neurodegenerative disease and the leading cause of dementia among senile subjects. It has been proposed that AD can be caused by defects in mitochondrial oxidative phosphorylation. Given the fundamental contribution of the mitochondrial genome (mtDNA) for the respiratory chain, there have been a number of studies investigating the association between mtDNA inherited variants and multifactorial diseases, however no general consensus has been reached yet on the correlation between mtDNA haplogroups and AD.Methodology/principal findingsWe applied for the first time a high resolution analysis (sequencing of displacement loop and restriction analysis of specific markers in the coding region of mtDNA) to investigate the possible association between mtDNA-inherited sequence variation and AD in 936 AD patients and 776 cognitively assessed normal controls from central and northern Italy. Among over 40 mtDNA sub-haplogroups analysed, we found that sub-haplogroup H5 is a risk factor for AD (OR=1.85, 95% CI:1.04-3.23) in particular for females (OR=2.19, 95% CI:1.06-4.51) and independently from the APOE genotype. Multivariate logistic regression revealed an interaction between H5 and age. When the whole sample is considered, the H5a subgroup of molecules, harboring the 4336 transition in the tRNAGln gene, already associated to AD in early studies, was about threefold more represented in AD patients than in controls (2.0% vs 0.8%; p=0.031), and it might account for the increased frequency of H5 in AD patients (4.2% vs 2.3%). The complete re-sequencing of the 56 mtDNAs belonging to H5 revealed that AD patients showed a trend towards a higher number (p=0.052) of sporadic mutations in tRNA and rRNA genes when compared with controls.ConclusionsOur results indicate that high resolution analysis of inherited mtDNA sequence variation can help in identifying both ancient polymorphisms defining sub-haplogroups and the accumulation of sporadic mutations associated with complex traits such as AD.
- Subjects :
- INVOLVEMENT
SELECTION
Mitochondrial DNA
CYTOCHROME-C-OXIDASE
mtDNA
Alzheimer's disease
Science
Respiratory chain
Biology
Genetics and Genomics/Complex Traits
VARIANTS
Human mitochondrial genetics
DNA, Mitochondrial
Haplogroup
Alzheimer Disease
HUMAN-POPULATIONS
medicine
Dementia
Humans
EPIDEMIOLOGY
Genetic Predisposition to Disease
LONGEVITY
Phylogeny
Aged
Genetics
Multidisciplinary
Polymorphism, Genetic
MUTATIONS
Haplotype
HUMAN MTDNA
NEAR-EASTERN
medicine.disease
Evolutionary Biology/Human Evolution
Haplotypes
Case-Control Studies
Mutation
Medicine
Female
Public Health and Epidemiology/Epidemiology
Neurological Disorders/Alzheimer Disease
Human mitochondrial DNA haplogroup
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, Vol 5, Iss 8, p e12037 (2010)
- Accession number :
- edsair.doi.dedup.....0494467e1a587a99e13c49988e16acc1