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Characterization of immobilized nuclease P1
- Source :
- Applied biochemistry and biotechnology. 136(1)
- Publication Year :
- 2006
-
Abstract
- To improve the efficiency of the use of nuclease P1, enzyme immobilization technology was applied using nuclease P1. Characterization of immobilized nuclease P1 on different supports was studied. The results showed that the optimum pH and temperature of nuclease P1 immobilized via different supports were enhanced. The immobilized enzyme was obviously stable when stored for long periods and was reusable. The best results were obtained when nuclease P1 was immobilized on chitosan nanoparticles. The nanoparticles were applied to protect the activity of nuclease P1 and improved enzyme activity by 13.17% over that of free nuclease P1 at the same conditions. The Michaelis constant Km and Vmax were determined for free and immobilized enzyme as well.
- Subjects :
- Hot Temperature
Immobilized enzyme
Bioengineering
Applied Microbiology and Biotechnology
Biochemistry
Michaelis–Menten kinetics
Chitosan
chemistry.chemical_compound
Enzyme Stability
Cellulose
Molecular Biology
chemistry.chemical_classification
Nuclease
Chromatography
biology
Single-Strand Specific DNA and RNA Endonucleases
Temperature
General Medicine
Hydrogen-Ion Concentration
Enzymes, Immobilized
Enzyme assay
Enzyme
chemistry
biology.protein
Aspergillus nuclease S1
Nanoparticles
Biotechnology
Subjects
Details
- ISSN :
- 02732289
- Volume :
- 136
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Applied biochemistry and biotechnology
- Accession number :
- edsair.doi.dedup.....04a8f67071e7acc4a86025cb8f2dd013