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Knockdown of SLC39A7 inhibits cell growth and induces apoptosis in human colorectal cancer cells
- Source :
- Acta Biochimica et Biophysica Sinica. 49:926-934
- Publication Year :
- 2017
- Publisher :
- China Science Publishing & Media Ltd., 2017.
-
Abstract
- SLC39A7 (zip7) is a zinc transporter that plays a key role in intestinal epithelial self-renewal. However, little is known about SLC39A7 in colorectal cancer. To assess the biological function of SLC39A7 in colorectal cancer, the expression of SLC39A7 in human colorectal tumors and five colorectal cancer cell lines were evaluated by Oncomine Cancer Microarray Database and western blot analysis. In addition, short hairpin RNAs specifically targeting SLC39A7 were transfected into HCT116 and SW1116 cells to knockdown SLC39A7 expression. Then, the effects of SLC39A7 knockdown on colorectal cancer cells were detected by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide, colony-forming assay and flow cytometry. Our results showed that colorectal tumors have higher expression levels of SLC39A7 than normal colon tissues. Knockdown of SLC39A7 exhibited a significant decrease in cell viability and proliferation of colorectal cancer cells. It was also shown that knockdown of SLC39A7 interfered with cell cycle progression and induced G2/M cell cycle arrest, as well as boosted early and late apoptosis in colorectal cancer cells. Furthermore, downregulation of SLC39A7 promoted the cleavage of PARP and enhanced the expression of Bad, Caspase-9, and cleaved-Caspase-3, as well as suppressed Bcl-2 expression. In conclusion, our results suggest that SLC39A7 plays a crucial role in the proliferation and survival of colorectal cancer cells, which associates with colorectal tumorigenesis.
- Subjects :
- 0301 basic medicine
Cell Survival
Colorectal cancer
Biophysics
Apoptosis
Biology
Biochemistry
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
medicine
Humans
Viability assay
Cation Transport Proteins
Cell Proliferation
Gene knockdown
Cell growth
HEK 293 cells
General Medicine
Transfection
HCT116 Cells
medicine.disease
Gene Expression Regulation, Neoplastic
HEK293 Cells
030104 developmental biology
Cell culture
030220 oncology & carcinogenesis
Cancer research
RNA Interference
Apoptosis Regulatory Proteins
Colorectal Neoplasms
Subjects
Details
- ISSN :
- 16729145
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Acta Biochimica et Biophysica Sinica
- Accession number :
- edsair.doi.dedup.....04b7f7839d555a1faefb7aec968c4d47
- Full Text :
- https://doi.org/10.1093/abbs/gmx094