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Shigella flexneri 2a strain 2457T expresses three members of the H-NS-like protein family: characterization of the Sfh protein
- Source :
- Molecular Genetics and Genomics, Molecular Genetics and Genomics, 2003, 270 (1), pp.66-77. ⟨10.1007/s00438-003-0897-0⟩, Molecular Genetics and Genomics, Springer Verlag, 2003, 270 (1), pp.66-77. ⟨10.1007/s00438-003-0897-0⟩
- Publication Year :
- 2003
-
Abstract
- Shigella flexneri 2a is known to express the H-NS nucleoid-structuring protein and the paralogous protein StpA. Using bioinformatic analysis we have now discovered a third member of the H-NS protein family, Sfh (S higella f lexneri H-NS-like protein), in strain 2457T. This protein is encoded by the sfh gene, which is located on a high-molecular-mass plasmid that is closely related to the self-transmissible plasmid R27. When expressed in Escherichia coli, the Sfh protein can complement an hns null mutation, restoring wild-type Bgl, porin protein, and mucoidy phenotypes, and wild-type expression of the fliC and proU genes. While a knockout mutation in the sfh gene alone had no effect on the expression of virulence genes in S. flexneri, an additive effect on virulence gene derepression was seen when the sfh lesion was combined with a mutation in hns. Over-expression of the sfh gene repressed expression of the VirB virulence regulatory protein and transcription of a VirB-dependent structural gene promoter. The purified Sfh protein bound specifically to DNA sequences containing the promoters of the virF and virB virulence regulatory genes. These findings show that Sfh has the ability to influence genetic events beyond the genetic element that encodes it, including the expression of the S. flexneri virulence genes. They raise the possibility of a triangular relationship among three closely related proteins with broad consequences for genetic events in the bacterium that harbours them.
- Subjects :
- MESH: Operon
MESH: Shigella flexneri
Protein family
[SDV]Life Sciences [q-bio]
Virulence
Plasmid R27
Shigella flexneri
H-NS protein
03 medical and health sciences
Plasmid
Bacterial Proteins
MESH: Plasmids
Operon
Genetics
Escherichia coli
MESH: Phylogeny
MESH: Bacterial Proteins
Molecular Biology
Gene
Phylogeny
030304 developmental biology
Regulator gene
Regulation of gene expression
MESH: Gene Expression Regulation, Bacterial
0303 health sciences
biology
MESH: Escherichia coli
030306 microbiology
Structural gene
Sfh protein
General Medicine
Gene Expression Regulation, Bacterial
biology.organism_classification
Molecular biology
DNA-Binding Proteins
StpA protein
MESH: DNA-Binding Proteins
Plasmids
Subjects
Details
- ISSN :
- 16174615 and 16174623
- Volume :
- 270
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Molecular genetics and genomics : MGG
- Accession number :
- edsair.doi.dedup.....04c1838accc001cf6959fc6059aef6d5