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Interferon γ increases sensitivity to endotoxin
Interferon γ increases sensitivity to endotoxin
- Source :
- Journal of Surgical Research. 51:197-203
- Publication Year :
- 1991
- Publisher :
- Elsevier BV, 1991.
-
Abstract
- Interferon-γ (IFN-γ) has been proposed for use following severe trauma to reverse depressed macrophage (Mφ) function and thereby reduce infection, sepsis, and subsequent multiple organ failure syndrome (MOFS). However, an excessive inflammatory response by Mφs and other components of the inflammatory cascade is thought to be central to the underlying pathophysiology of MOFS. Endotoxin (LPS) has been implicated as a principal mediator of sepsis-induced MOFS by stimulating Mφs and leukocytes (WBC). This study addresses the following question: Does IFN-γ predispose normal rabbits to a pathophysiologic response to LPS infusion? Four groups of New Zealand White rabbits ( n = 6, each group) were prepared for measurement of cardiac output, arterial pressure, arterial PO 2 , and WBC counts over a 6-hr period. Group I (control) was instrumented alone, Group II (LPS alone) was given a subclinical dose of 1.0 μg/kg of Escherichia coli LPS iv, Group III (IFN-γ alone) was given recombinant rabbit IFN-γ (5.0 μg/kg subcutaneous) for 3 days prior to preparation for measurements, and Group IV (IFN-γ + LPS) received 3 days of IFN-γ followed by LPS. One hour prior to sacrifice 5.0 μCi of 125 I-albumin was given and bronchoalveolar lavage was performed at death to determine the lavage/plasma 125 I ratio as an index of pulmonary permeability. The results indicate that IFN + LPS animals had significant decreases in cardiac output, PO 2 , and WBC counts, and increased lavage/plasma ratio of 125 I-albumin when compared to all other groups ( P t test). Neither LPS alone nor IFN-γ alone had a significant effect on measured variables. In contrast to immunosuppressed animals, IFN-γ pretreatment of normal animals increases the host pathophysiologic response to endotoxin.
- Subjects :
- Lipopolysaccharides
Pulmonary Circulation
medicine.medical_specialty
Cardiac output
Blood Pressure
Capillary Permeability
Sepsis
Interferon-gamma
Leukocyte Count
Interferon
Internal medicine
Escherichia coli
medicine
Animals
Macrophage
Interferon gamma
Cardiac Output
medicine.diagnostic_test
business.industry
Arteries
medicine.disease
Pathophysiology
Endotoxins
Oxygen
Endocrinology
Blood pressure
Bronchoalveolar lavage
Immunology
Surgery
Rabbits
business
medicine.drug
Subjects
Details
- ISSN :
- 00224804
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Journal of Surgical Research
- Accession number :
- edsair.doi.dedup.....04f72836e21aee73f8537855c5a34631
- Full Text :
- https://doi.org/10.1016/0022-4804(91)90094-3