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The GEN.S: a fortuitous finding of a routine screening test for hereditary spherocytosis

Authors :
Thérèse Cynober
F Mielot
Gil Tchernia
M. Chiron
L. Croisille
Source :
Hematology and Cell Therapy. 41:113-116
Publication Year :
1999
Publisher :
Springer Science and Business Media LLC, 1999.

Abstract

As part of the evaluation of the GEN.S (Coulter), we compared the Mean Corpuscular Volume (MCV) to the Mean Spherized Corpuscular Volume (MSCV) assessed during the reticulocyte count procedure under hypo-osmotic conditions. A sub-group of patients with hereditary spherocytosis (HS) was singled out: in all of them, the MSCV became smaller than the MCV. As the cell volume normally increases in red cells derived from other patients in the same conditions, we decided to further study the reason for this particular behaviour of HS red cells. Whereas normal red cells are able to undergo an osmotic expansion, the spherocytes reach a critical osmotic volume leading to cell fragmentation consistent with the decrease of MSCV. This fortuitous finding is likely to be a reliable improvement for the routine screening of HS.

Details

ISSN :
12798509 and 12693286
Volume :
41
Database :
OpenAIRE
Journal :
Hematology and Cell Therapy
Accession number :
edsair.doi.dedup.....0560a03f2079be57a643c35a278739d7
Full Text :
https://doi.org/10.1007/s00282-999-0113-8