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The GEN.S: a fortuitous finding of a routine screening test for hereditary spherocytosis
- Source :
- Hematology and Cell Therapy. 41:113-116
- Publication Year :
- 1999
- Publisher :
- Springer Science and Business Media LLC, 1999.
-
Abstract
- As part of the evaluation of the GEN.S (Coulter), we compared the Mean Corpuscular Volume (MCV) to the Mean Spherized Corpuscular Volume (MSCV) assessed during the reticulocyte count procedure under hypo-osmotic conditions. A sub-group of patients with hereditary spherocytosis (HS) was singled out: in all of them, the MSCV became smaller than the MCV. As the cell volume normally increases in red cells derived from other patients in the same conditions, we decided to further study the reason for this particular behaviour of HS red cells. Whereas normal red cells are able to undergo an osmotic expansion, the spherocytes reach a critical osmotic volume leading to cell fragmentation consistent with the decrease of MSCV. This fortuitous finding is likely to be a reliable improvement for the routine screening of HS.
- Subjects :
- Adult
Erythrocyte Indices
Hemolytic anemia
medicine.medical_specialty
Pathology
Adolescent
Cell volume
Spherocytosis, Hereditary
Hereditary spherocytosis
Andrology
Reticulocyte Count
Reticulocyte
Osmotic Pressure
Internal medicine
medicine
Humans
Child
Mean corpuscular volume
Routine screening
Hematology
medicine.diagnostic_test
business.industry
medicine.disease
medicine.anatomical_structure
Hypotonic Solutions
Reticulocyte count (procedure)
business
circulatory and respiratory physiology
Subjects
Details
- ISSN :
- 12798509 and 12693286
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- Hematology and Cell Therapy
- Accession number :
- edsair.doi.dedup.....0560a03f2079be57a643c35a278739d7
- Full Text :
- https://doi.org/10.1007/s00282-999-0113-8