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Ribosomal protein S27-like in colorectal cancer: a candidate for predicting prognoses
- Source :
- PLoS ONE, PLoS ONE, Vol 8, Iss 6, p e67043 (2013)
- Publication Year :
- 2013
-
Abstract
- Background: The development and progression of colorectal cancer (CRC) involve a complex process of multiple genetic changes. Tumor suppressor p53 is capable of determining the fate of CRC cells. However, the role of a p53-inducible modulator, ribosomal protein S27-like (RPS27L), in CRC is unknown. Methods: Here, the differential expression of RPS27L was examined in the feces and colonic tissues of CRC patients, to explore its possible correlation with patient survival and to investigate the cellular mechanisms underlying their clinical outcomes. Eighty intermediate-stage CRC patients (42 at stage II and 38 at stage III) were divided into two groups according to their fecal RPS27L mRNA levels. The survival probabilities of the groups were estimated using the Kaplan–Meier method. The RPS27L protein in the colonic tissues of stage III patients with different prognoses was further examined immunohistochemically. RPS27L expression in LoVo cells was manipulated to examine the possible cellular responses in vitro. Results: Elevated RPS27L expression, in either feces or tissues, was related to a better prognosis. In vitro, RPS27L-expressing LoVo cells ceased DNA synthesis and apoptotic activity while the expression of their DNA repair molecules was upregulated. Conclusions: Elevated RPS27L may improve the prognoses of certain CRC patients by enhancing the DNA repair capacity of their colonic cells, and can be determined in feces. By integrating clinical, molecular, and cellular data, our study demonstrates that fecal RPS27L may be a useful index for predicting prognoses and guiding personalized therapeutic strategies, especially in patients with intermediate-stage CRC.
- Subjects :
- Male
DNA Repair
Colorectal cancer
lcsh:Medicine
Bioinformatics
Feces
Molecular cell biology
Basic Cancer Research
DNA Breaks, Double-Stranded
lcsh:Science
Regulation of gene expression
Multidisciplinary
Cell Death
Cancer Risk Factors
Colon Adenocarcinoma
Nuclear Proteins
RNA-Binding Proteins
Prognosis
Gene Expression Regulation, Neoplastic
Nucleic acids
Protein Transport
Oncology
Medicine
Female
Colorectal Neoplasms
Cancer Screening
Research Article
Ribosomal Proteins
DNA repair
DNA damage
Genetic Causes of Cancer
Down-Regulation
Biology
Rectal Cancer
Downregulation and upregulation
Cell Line, Tumor
Metalloproteins
Gastrointestinal Tumors
medicine
Genetics
Cancer Genetics
Cancer Detection and Diagnosis
Humans
Survival analysis
Aged
Proportional Hazards Models
Clinical Genetics
lcsh:R
Cancers and Neoplasms
DNA
medicine.disease
Survival Analysis
In vitro
Apoptosis
Cancer research
lcsh:Q
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....0575e25188a5b48ee318de944628692f