Additional file 1 of Minimization and optimization of α-amylase terminator for heterologous protein production in Bacillus licheniformis

Additional file1: Table S1. Strains and plasmids used in this study. Table S2. Primers used in this study. Table S3. The effects of GC content at the bottom of stem-loop structure, Gibbs free energy ΔG, U-tract length on terminator performances. Table S4. The sequences of different terminators plasmids used in this study. Table S5. Comparison of termination efficiency of different terminators in Bacillus. Figure S1. The secondary structure map of TamyL with a size of 501 bp. Figure S2. The construction map of plasmids with different terminators and dual reporter genes.