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Physiological and metabolic consequences of autophagy deficiency for the management of nitrogen and protein resources in Arabidopsis leaves depending on nitrate availability
- Source :
- New Phytologist, New Phytologist, Wiley, 2013, 199 (3), pp.683-694, New Phytologist, Wiley, 2013, 199 (3), pp.683-694. ⟨10.1111/nph.12307⟩
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- Article de revue (Article scientifique dans une revue à comité de lecture); International audience; Autophagy is present at a basal level in all plant tissues and is induced during leaf ageing and in response to nitrogen (N) starvation. Nitrogen remobilization from the rosette to the seeds is impaired in autophagy mutants. This report focuses on the role of autophagy in leaf N management and proteolysis during plant ageing.Metabolites, enzyme activities and protein contents were monitored in several autophagy-defective (atg) Arabidopsis mutants grown under low and high nitrate conditions.Results showed that carbon (C) and N statuses were affected in atg mutants before any senescence symptoms appeared. atg mutants accumulated larger amounts of ammonium, amino acids and proteins than wild type, and were depleted in sugars. Over-accumulation of proteins in atg mutants was selective and occurred despite higher endopeptidase and carboxypeptidase activities. Specific over-accumulation of the ribosomal proteins S6 and L13 subunits, and of catalase and glutamate dehydrogenase proteins was observed. atg mutants also accumulated peptides putatively identified as degradation products of the Rubisco large subunit and glutamine synthetase 2 (GS2). Incomplete chloroplast protein degradation resulting from autophagy defects could explain the higher N concentrations measured in atg rosettes and defects in N remobilization.It is concluded that autophagy controls C:N status and protein content in leaves of Arabidopsis.
- Subjects :
- 0106 biological sciences
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
Physiology
leaf senescence
Arabidopsis
Plant Science
01 natural sciences
AMINO-ACIDS
Biomass
2. Zero hunger
0303 health sciences
aminopeptidase
biology
medicine.diagnostic_test
PROFILING APPROACH
nitrogen remobilization
REMOBILIZATION
carboxypeptidase
Biochemistry
5-BISPHOSPHATE CARBOXYLASE OXYGENASE
Carbohydrate Metabolism
RNA Interference
RIBULOSE-1
EUGLENA-GRACILIS
nitrate availability
Nitrogen
Proteolysis
Blotting, Western
Protein degradation
03 medical and health sciences
Ribosomal protein
Glutamate-Ammonia Ligase
GLUTAMINE-SYNTHETASE
Glutamine synthetase
medicine
Autophagy
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
PLANTS
030304 developmental biology
selective autophagy
SENESCING LEAVES
Nitrates
Arabidopsis Proteins
Glutamate dehydrogenase
RuBisCO
DEGRADATION
biology.organism_classification
Carbon
Plant Leaves
Mutation
biology.protein
010606 plant biology & botany
Peptide Hydrolases
Subjects
Details
- Language :
- English
- ISSN :
- 0028646X and 14698137
- Database :
- OpenAIRE
- Journal :
- New Phytologist, New Phytologist, Wiley, 2013, 199 (3), pp.683-694, New Phytologist, Wiley, 2013, 199 (3), pp.683-694. ⟨10.1111/nph.12307⟩
- Accession number :
- edsair.doi.dedup.....05d76d3c8ab4056b0979362c4d8290ac