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Peripheral Blood Stem Cell Mobilization in Healthy Donors by Granulocyte Colony-Stimulating Factor Causes Preferential Mobilization of Lymphocyte Subsets
- Source :
- Frontiers in Immunology, Vol 9 (2018), Frontiers in Immunology, 9:845, MAY
- Publication Year :
- 2018
- Publisher :
- Frontiers Media S.A., 2018.
-
Abstract
- Background: Allogeneic hematopoietic stem cell transplantation is associated with a high risk of immune-mediated post-transplant complications. Graft depletion of immunocompetent cell subsets is regarded as a possible strategy to reduce this risk without reducing antileukemic immune reactivity. Study design and methods: We investigated the effect of hematopoietic stem cell mobilization with granulocyte colony-stimulating factor (G-CSF) on peripheral blood and stem cell graft levels of various T, B, and NK cell subsets in healthy donors. The results from flow cytometric cell quantification were examined by bioinformatics analyses. Results: The G-CSF-induced mobilization of lymphocytes was a non-random process with preferential mobilization of naïve CD4+ and CD8+ T cells together with T cell receptor αβ+ T cells, naïve T regulatory cells, type 1 T regulatory cells, mature and memory B cells, and cytokine-producing NK cells. Analysis of circulating lymphoid cell capacity to release various cytokines (IFNγ, IL10, TGFβ, IL4, IL9, IL17, and IL22) showed preferential mobilization of IL10 releasing CD4+ T cells and CD3−19− cells. During G-CSF treatment, the healthy donors formed two subsets with generally strong and weaker mobilization of immunocompetent cells, respectively; hence the donors differed in their G-CSF responsiveness with regard to mobilization of immunocompetent cells. The different responsiveness was not reflected in the graft levels of various immunocompetent cell subsets. Furthermore, differences in donor G-CSF responsiveness were associated with time until platelet engraftment. Finally, strong G-CSF-induced mobilization of various T cell subsets seemed to increase the risk of recipient acute graft versus host disease, and this was independent of the graft T cell levels. Conclusion: Healthy donors differ in their G-CSF responsiveness and preferential mobilization of immunocompetent cells. This difference seems to influence post-transplant recipient outcomes. publishedVersion
- Subjects :
- Male
medicine.medical_treatment
Graft vs Host Disease
apheresis
Hematopoietic stem cell transplantation
CD8-Positive T-Lymphocytes
0302 clinical medicine
living donors
T-Lymphocyte Subsets
graft versus host disease
Immunology and Allergy
Medicine
Original Research
peripheral blood stem cells
immune reconstitution
Middle Aged
Allografts
Healthy Volunteers
Tissue Donors
Granulocyte colony-stimulating factor
Killer Cells, Natural
Interleukin 10
medicine.anatomical_structure
030220 oncology & carcinogenesis
hematopoietic stem cell transplantation
Blood Component Removal
Cytokines
Female
Stem cell
granulocyte colony-stimulating factor
Adult
lcsh:Immunologic diseases. Allergy
Filgrastim
T cell
Immunology
03 medical and health sciences
Hematologic Agents
Humans
Transplantation, Homologous
Hematopoietic Stem Cell Mobilization
Aged
business.industry
Computational Biology
medicine.disease
Graft-versus-host disease
hematopoietic stem cell mobilization
business
lcsh:RC581-607
CD8
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 16643224
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Frontiers in Immunology
- Accession number :
- edsair.doi.dedup.....05f223a01f4c3e64ec64e9cedef60746