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Genetic Selection Based on a Ste6*C-HA-Ura3 Substrate Identifies New Cytosolic Quality Control Alleles in Saccharomyces cerevisiae
- Source :
- G3: Genes, Genomes, Genetics, 10 (6), G3: Genes, Genomes, Genetics, Vol 10, Iss 6, Pp 1879-1891 (2020), G3: Genes|Genomes|Genetics
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- Protein quality control in the cytosol (CytoQC) is an important cellular pathway consisting of a network of components which monitor the folding of cytosolic proteins and ensure the efficient removal of aberrant ones. Our understanding of CytoQC mechanisms is limited in part by the ability of current approaches to identify new genes in the pathway. In this study, we developed a CytoQC reporter substrate, Ste6*C-HA-Ura3, for a new genetic selection of spontaneous CytoQC mutations in the yeast Saccharomyces cerevisiae. In addition to UBR1, which encodes for a known CytoQC E3 ligase, we identified six new CytoQC candidates. In the preliminary characterization of two mutants, we found that Doa4 is involved in the degradation of misfolded substrates while Pup2 functions in the selectivity of CytoQC and ERAD substrates. Overall, the strategy demonstrates the potential to identify novel genes and advance our understanding of CytoQC.<br />G3: Genes, Genomes, Genetics, 10 (6)<br />ISSN:2160-1836
- Subjects :
- Saccharomyces cerevisiae Proteins
Ubiquitin-Protein Ligases
Mutant
Saccharomyces cerevisiae
Computational biology
QH426-470
Endoplasmic-reticulum-associated protein degradation
03 medical and health sciences
Cytosol
0302 clinical medicine
Genetics
URA3
Selection, Genetic
Molecular Biology
Gene
Alleles
Genetics (clinical)
030304 developmental biology
genetic selection
cytosolic protein quality control
spontaneous mutations
0303 health sciences
biology
Mutant Screen Report
biology.organism_classification
Yeast
Ubiquitin ligase
biology.protein
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 21601836
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- G3 Genes|Genomes|Genetics
- Accession number :
- edsair.doi.dedup.....0609c2b3c33f8ab216a3d62f4703fbf6