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Protection of pigs against genital Chlamydia trachomatis challenge by parenteral or mucosal DNA immunization
- Source :
- Vaccine. 30:2869-2881
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- The current study evaluates combined aerosol-vaginal delivery of a MOMP-based Chlamydia trachomatis (serovar E) DNA vaccine in a pig genital challenge model. Most non-replicating antigens are rather poor mucosal immunogens in comparison to replicating antigens. Therefore, a mucosal administered DNA vaccine, which actually mimics a live vaccine, could be promising. Protection was promoted by plasmids encoding the porcine granulocyte macrophage-colony stimulating factor (pcDNA3.1zeo::GM-CSF), the Escherichia coli thermo-labile enterotoxin (LT) subunit A (plasmid PJV2004::LTa) and subunit B (plasmid PJV2005::LTb). Mucosal C. trachomatis DNA vaccination induced significant protection against genital C. trachomatis challenge although the infection could not be eradicated. Intradermal immunization was significantly less efficient in protecting experimentally infected pigs. Protection was correlated with efficient T cell priming and significantly higher serum IgA titers following primo vaccination.
- Subjects :
- Serotype
Swine
Chlamydia trachomatis
Enterotoxin
Biology
medicine.disease_cause
DNA vaccination
Microbiology
Plasmid
Adjuvants, Immunologic
Antigen
Administration, Inhalation
Vaccines, DNA
medicine
Animals
Immunity, Mucosal
Attenuated vaccine
General Veterinary
General Immunology and Microbiology
Public Health, Environmental and Occupational Health
Virology
Vaccination
Administration, Intravaginal
Disease Models, Animal
Infectious Diseases
Bacterial Vaccines
Lymphogranuloma Venereum
Molecular Medicine
Female
Immunization
Plasmids
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....060f62345361a2f8c1d2eeebc082d59f