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Chemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors
- Source :
- International Journal of Molecular Sciences, Volume 22, Issue 22, International Journal of Molecular Sciences, Vol 22, Iss 12150, p 12150 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Chemoattraction is a normal and essential process, but it can also be involved in tumorigenesis. This phenomenon plays a key role in glioblastoma (GBM). The GBM tumor cells are extremely difficult to eradicate, due to their strong capacity to migrate into the brain parenchyma. Consequently, a complete resection of the tumor is rarely a possibility, and recurrence is inevitable. To overcome this problem, we proposed to exploit this behavior by using three chemoattractants: CXCL10, CCL2 and CCL11, released by a biodegradable hydrogel (GlioGel) to produce a migration of tumor cells toward a therapeutic trap. To investigate this hypothesis, the agarose drop assay was used to test the chemoattraction capacity of these three chemokines on murine F98 and human U87MG cell lines. We then studied the potency of this approach in vivo in the well-established syngeneic F98-Fischer glioma-bearing rat model using GlioGel containing different mixtures of the chemoattractants. In vitro assays resulted in an invasive cell rate 2-fold higher when chemokines were present in the environment. In vivo experiments demonstrated the capacity of these specific chemoattractants to strongly attract neoplastic glioblastoma cells. The use of this strong locomotion ability to our end is a promising avenue in the establishment of a new therapeutic approach in the treatment of primary brain tumors.
- Subjects :
- Chemokine CCL11
Male
Chemokine
QH301-705.5
Cell
Brain tumor
CCL2
Biology
medicine.disease_cause
migration
Article
Catalysis
Inorganic Chemistry
Mice
In vivo
Cell Line, Tumor
medicine
Animals
Humans
CXCL10
Biology (General)
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
Chemokine CCL2
Spectroscopy
Brain Neoplasms
Organic Chemistry
chemokine
glioblastoma
Chemotaxis
General Medicine
medicine.disease
Rats, Inbred F344
Neoplasm Proteins
Rats
Computer Science Applications
Chemokine CXCL10
Chemistry
medicine.anatomical_structure
Cancer research
biology.protein
Carcinogenesis
Neuroglia
neuro-oncology
brain tumor
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....0630d3a162dbecf6d948161ad6560f87
- Full Text :
- https://doi.org/10.3390/ijms222212150