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The G-protein-coupled receptor APJ is expressed in the second heart field and regulates Cerberus–Baf60c axis in embryonic stem cell cardiomyogenesis
- Source :
- Cardiovascular research (2013). doi:10.1093/cvr/cvt166, info:cnr-pdr/source/autori:D'Aniello C, Fiorenzano A, Iaconis S, Liguori GL, Andolfi G, Cobellis G, Fico A, Minchiotti G./titolo:The G protein coupled receptor Apj is expressed in the second heart field and regulates Cerberus-Baf60C axis in embryonic stem cell cardiomyogenesis./doi:10.1093%2Fcvr%2Fcvt166/rivista:Cardiovascular research/anno:2013/pagina_da:/pagina_a:/intervallo_pagine:/volume, Cardiovascular Research; Vol 100
- Publication Year :
- 2013
- Publisher :
- Oxford University Press (OUP), 2013.
-
Abstract
- Aims Mammalian cardiomyogenesis occurs through a multistep process that requires a complex network of tightly regulated extracellular signals, which integrate with the genetic and epigenetic machinery to maintain, expand, and regulate the differentiation of cardiac progenitor cells. Pluripotent embryonic stem cells (ESCs) recapitulate many aspects of development, and have provided an excellent opportunity to dissect the molecular mechanisms underlying cardiomyogenesis, which is still incompletely defined. Methods and results We provide new in vivo evidence that the G-protein-coupled receptor angiotensin receptor-like 1 ( Apj ) is expressed in the mesodermal cells of the second heart field, a population of cardiac progenitors that give rise to a major part of the definitive heart. By combining loss-and-gain of function studies in mouse ESCs, we show that Apj (i) controls the balance between proliferation and cardiovascular differentiation, (ii) regulates the Nodal/Bone Morphogenetic Protein antagonist Cerberus and the Baf60c/Smarcd3 subunit of the Brg1/Brm-associated factors (BAF) chromatin-remodelling complex. Conclusion We propose a model in which Apj controls a regulatory Cerberus–Baf60c pathway in pluripotent stem cell cardiomyogenesis, and speculate that this regulatory circuit may regulate cardiac progenitor cell behaviour.
- Subjects :
- Chromosomal Proteins, Non-Histone
Physiology
Second heart field
Muscle Proteins
Smad2 Protein
Receptors, G-Protein-Coupled
Mice
0302 clinical medicine
Myocytes, Cardiac
Induced pluripotent stem cell
Cells, Cultured
Genetics
Apelin Receptors
0303 health sciences
education.field_of_study
Stem cell
Epigenetic
Cell Differentiation
Heart
Cell biology
Myocardial progenitor
Bone Morphogenetic Proteins
embryonic structures
Cytokines
Apelin Receptor
Cardiology and Cardiovascular Medicine
Signal Transduction
G-protein-coupled receptor
Nodal Protein
Population
Muscle Protein
Biology
Bone morphogenetic protein
03 medical and health sciences
Cerberus (protein)
Embryonic Stem Cell
Physiology (medical)
Animals
education
Cyclin-Dependent Kinase Inhibitor p57
Embryonic Stem Cells
Cell Proliferation
030304 developmental biology
G protein-coupled receptor
Animal
Bone Morphogenetic Protein
Protein
Proteins
Embryonic stem cell
NODAL
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 17553245 and 00086363
- Volume :
- 100
- Database :
- OpenAIRE
- Journal :
- Cardiovascular Research
- Accession number :
- edsair.doi.dedup.....06ae5e0fcff3f8328853f147cb4e969d