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Immunostimulant and biocompatible gold and silver nanoparticles synthesized using the Ulva intestinalis L. aqueous extract
- Source :
- Journal of Materials Chemistry B 7 (2019): 4677–4691. doi:10.1039/c9tb00215d, info:cnr-pdr/source/autori:Gonzalez-Ballesteros N.; Diego-Gonzalez L.; Lastra-Valdor M.; Rodriguez-Arguelles M.C.; Grimaldi M.; Cavazza A.; Bigi F.; Simon-Vazquez R./titolo:Immunostimulant and biocompatible gold and silver nanoparticles synthesized using the: Ulva intestinalis L. aqueous extract/doi:10.1039%2Fc9tb00215d/rivista:Journal of Materials Chemistry B/anno:2019/pagina_da:4677/pagina_a:4691/intervallo_pagine:4677–4691/volume:7
- Publication Year :
- 2019
- Publisher :
- Royal Society of Chemistry (RSC), 2019.
-
Abstract
- This is the first study to report on the biocompatible and immunogenic properties of one-pot synthesised gold and silver nanoparticles (Au@UI and Ag@UI) using the macroalgae Ulva intestinalis (UI). The UI aqueous extract, Au@UI, and Ag@UI were obtained under sterile conditions and fully characterized by UV-vis spectroscopy, TEM, HRTEM, STEM and FTIR spectroscopy. Moreover, for the first time, the composition of carbohydrates in the UI extract has been reported along with the changes observed after nanoparticle synthesis by size exclusion chromatography, in order to investigate their possible role in the biosynthetic process. This study suggested that the polysaccharide fraction of the extract is involved in the formation and stabilization of the nanoparticles. The potential toxicity of the samples was evaluated using different cell lines and the hemocompatibility was tested in mouse erythrocytes. In addition, ROS production, complement activation and cytokine release were evaluated to determine the immunogenicity. The results showed that Au@UI and Ag@UI exhibit good biocompatibility and hemocompatibility, with the exception of Ag@UI nanoparticles at high concentration, which were hemolytic. The samples induced ROS release and complement activation, two key mechanisms in innate immunity. The samples also induced the release of cytokines from Th1 and Th2 profiles, and other cytokines implicated in the activation of the immune system. Au@UI and Ag@UI were biocompatible and preserved the immunostimulant properties of the UI extract. Hence, Au@UI and Ag@UI could be useful as adjuvants in vaccine development and promote a balanced Th1 and Th2 immune response mediated by ROS production, cytokine release and complement activation.
- Subjects :
- Silver
Biocompatibility
medicine.drug_class
Biomedical Engineering
Metal Nanoparticles
Biocompatible Materials
02 engineering and technology
010402 general chemistry
Polysaccharide
01 natural sciences
Immunostimulant
Silver nanoparticle
Cell Line
Mice
Ulva
Th2 Cells
Immune system
Adjuvants, Immunologic
Chlorophyta
Polysaccharides
medicine
Animals
General Materials Science
Complement Activation
chemistry.chemical_classification
Innate immune system
Chemistry
GREEN SYNTHESIS
IN-VITRO
SULFATED POLYSACCHARIDES
COMPLEMENT ACTIVATION
VACCINE
ANTIOXIDANT
ANTIBACTERIAL
NANOMATERIALS
CYTOTOXICITY
ENTEROMORPHA
General Chemistry
General Medicine
Th1 Cells
021001 nanoscience & nanotechnology
Immunity, Innate
0104 chemical sciences
Complement system
Biochemistry
Biosynthetic process
Cytokines
Gold
Reactive Oxygen Species
0210 nano-technology
Subjects
Details
- ISSN :
- 20507518 and 2050750X
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Journal of Materials Chemistry B
- Accession number :
- edsair.doi.dedup.....06b9f53fbbd008994fd15ef06c7429c8