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73-kDa Heat Shock Cognate Protein Interacts Directly with P27Kip1, a Cyclin-Dependent Kinase Inhibitor, during G1/S Transition
- Source :
- Biochemical and Biophysical Research Communications. 257:340-343
- Publication Year :
- 1999
- Publisher :
- Elsevier BV, 1999.
-
Abstract
- Although heat shock proteins (HSPs) were discovered as inducible proteins by the physical stress to protect cells, recent evidence has suggested that HSPs are likely involved in cell cycle control under normal conditions without stress. In the present study, we demonstrated that 73hsc (heat shock cognate protein), which belongs to the HSP70 family of molecular chaperones, interacts with P27Kip1, an inhibitor of cyclin-dependent kinase, during G1/S transition. 73hsc was detected in the immunoprecipitates with anti-P27Kip1 antibody and, vice versa, P27Kip1 was present in the immunoprecipitates with anti-73hsc antibody by Western blotting using growth-stimulated rat thyroid FRTL-5 cells. This complex formation of 73hsc and P27Kip1 was cell cycle dependent and its maximum formation was observed at G1/S transition where the level of P27Kip1 dramatically decreased. ATP dissociated this complex formation in a dose-dependent manner. These data indicated that 73hsc might be involved in the cell cycle progression through the regulation of cell cycle regulators such as P27Kip1.
- Subjects :
- Time Factors
Blotting, Western
Thyroid Gland
Biophysics
Thyrotropin
Cell Cycle Proteins
HSP72 Heat-Shock Proteins
Biology
Biochemistry
Cell Line
S Phase
Adenosine Triphosphate
Cyclin-dependent kinase
Cyclins
Heat shock protein
Animals
HSP70 Heat-Shock Proteins
Insulin-Like Growth Factor I
Phosphorylation
Cell Cycle Protein
Molecular Biology
Heat-Shock Proteins
Cyclin-dependent kinase 1
Tumor Suppressor Proteins
Cyclin-dependent kinase 2
G1 Phase
HSC70 Heat-Shock Proteins
G1/S transition
Cell Biology
Precipitin Tests
Molecular biology
Cyclin-Dependent Kinases
Rats
Hsp70
Cell biology
Adenosine Diphosphate
Heat shock factor
biology.protein
Carrier Proteins
Microtubule-Associated Proteins
Cyclin-Dependent Kinase Inhibitor p27
Protein Binding
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 257
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....06cbc1bfbca5ce0d26332e17d827e1d8