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Single-Gene Detection and Karyotyping Using Small-Target Fluorescence in Situ Hybridization on Maize Somatic Chromosomes
- Source :
- Genetics. 175:1047-1058
- Publication Year :
- 2007
- Publisher :
- Oxford University Press (OUP), 2007.
-
Abstract
- Combined with a system for identifying each of the chromosomes in a genome, visualizing the location of individual genetic loci by fluorescence in situ hybridization (FISH) would aid in assembling physical and genetic maps. Previously, large genomic clones have been successfully used as FISH probes onto somatic chromosomes but this approach is complicated in species with abundant repetitive elements. In this study, repeat-free portions of sequences that were anchored to particular chromosomes including genes, gene clusters, large cDNAs, and portions of BACs obtained from public databases were used to label the corresponding physical location using FISH. A collection of probes that includes at least one marker on each chromosome in the maize complement was assembled, allowing a small-target karyotyping system to be developed. This set provides the foundation onto which additional loci could be added to strengthen further the ability to perform chromosomal identification in maize and its relatives. The probes were demonstrated to produce signals in several wild relatives of maize, including Zea luxurians, Z. diploperennis, and Tripsacum dactyloides.
- Subjects :
- Genetics
biology
medicine.diagnostic_test
Somatic cell
Hybridization probe
Chromosome Mapping
Chromosome
Karyotype
Investigations
Zea luxurians
Genes, Plant
biology.organism_classification
Zea mays
Genome
Chromosomes, Plant
Species Specificity
Karyotyping
medicine
DNA Probes
Gene
In Situ Hybridization, Fluorescence
DNA Primers
Fluorescence in situ hybridization
Subjects
Details
- ISSN :
- 19432631
- Volume :
- 175
- Database :
- OpenAIRE
- Journal :
- Genetics
- Accession number :
- edsair.doi.dedup.....06fba914c632d6d149865e98287f2fc0