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Large-scale survey of porcine endogenous retrovirus in Chinese miniature pigs
- Source :
- Comparative Immunology, Microbiology and Infectious Diseases. 31:367-371
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- We conducted a large-scale survey on the existence and expression status of porcine endogenous retrovirus (PERV) in seven breeds of Chinese miniature pigs. Genotyping of PERV was examined by PCR using type-specific primers according to the env genotyping method. The presence and expression status of viral gag, pol and env genes were further analyzed in Wuzhishan pigs (WZSP) and Bama minipigs (BMP). The results showed that PERV existed in all 348 genomic DNA samples. The genotype distribution was subtype A-74.43%, subtype B-95.40% and subtype C-30.46%. No expression of subtype C was found in WZSP and BMP. This research obtained an adequate level of information on the molecular epidemiology of PERV in China. The results indicated that it is possible to monitor pig herds for individuals with the lowest PERV prevalence, especially lacking PERV-C.
- Subjects :
- China
Genes, Viral
Swine
Xenotransplantation
medicine.medical_treatment
Immunology
Endogenous retrovirus
Biology
Microbiology
Virus
Genotype
medicine
Animals
Immunology and Allergy
Gene
Genotyping
Genetics
Molecular Epidemiology
General Veterinary
Molecular epidemiology
Endogenous Retroviruses
General Medicine
Virology
genomic DNA
Infectious Diseases
Swine, Miniature
Subjects
Details
- ISSN :
- 01479571
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Comparative Immunology, Microbiology and Infectious Diseases
- Accession number :
- edsair.doi.dedup.....06fcd145af33e5e5a93f1e99444c31eb
- Full Text :
- https://doi.org/10.1016/j.cimid.2007.06.004