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Activation of mTORC1 by leucine is potentiated by branched-chain amino acids and even more so by essential amino acids following resistance exercise
- Publication Year :
- 2016
- Publisher :
- Mittuniversitetet, Avdelningen för hälsovetenskap, 2016.
-
Abstract
- Protein synthesis is stimulated by resistance exercise and intake of amino acids, in particular leucine. Moreover, activation of mammalian target of rapamycin complex 1 (mTORC1) signaling by leucine is potentiated by the presence of other essential amino acids (EAA). However, the contribution of the branched-chain amino acids (BCAA) to this effect is yet unknown. Here we compare the stimulatory role of leucine, BCAA, and EAA ingestion on anabolic signaling following exercise. Accordingly, eight trained volunteers completed four sessions of resistance exercise during which they ingested either placebo, leucine, BCAA, or EAA (including the BCAA) in random order. Muscle biopsies were taken at rest, immediately after exercise, and following 90 and 180 min of recovery. Following 90 min of recovery the activity of S6 kinase 1 (S6K1) was greater than at rest in all four trials (Placebo37/46 was unaffected by supplementation, while that of Thr46 alone exhibited a pattern similar to that of S6K1, being 18% higher with EAA than BCAA. However, after 180 min of recovery this difference between EAA and BCAA had disappeared, although with both these supplements the increases were still higher than with leucine (40%, P < 0.05) and placebo (100%, P < 0.05). In summary, EAA ingestion appears to stimulate translation initiation more effectively than the other supplements, although the results also suggest that this effect is primarily attributable to the BCAA.
- Subjects :
- 0301 basic medicine
Blood Glucose
Male
Medicin och hälsovetenskap
Time Factors
Physiology
Biopsy
4E-BP1
Skeletal muscle
Cell Cycle Proteins
mTORC1
Medical and Health Sciences
0302 clinical medicine
Chain (algebraic topology)
Protein biosynthesis
Insulin
Phosphorylation
BCAA
chemistry.chemical_classification
TOR Serine-Threonine Kinases
Ribosomal Protein S6 Kinases, 70-kDa
S6K1
Healthy Volunteers
Amino acid
medicine.anatomical_structure
Treatment Outcome
Biochemistry
Leucine
Signal Transduction
Adult
030209 endocrinology & metabolism
P70-S6 Kinase 1
Biology
Mechanistic Target of Rapamycin Complex 1
03 medical and health sciences
medicine
Humans
Lactic Acid
Muscle, Skeletal
Adaptor Proteins, Signal Transducing
Resistance training
Resistance Training
Cell Biology
Phosphoproteins
030104 developmental biology
Eukaryotic Initiation Factor-4E
chemistry
Multiprotein Complexes
Protein Biosynthesis
Amino Acids, Essential
Energy Metabolism
Amino Acids, Branched-Chain
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....0701491d842eb66cc74506a9f62437cd