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Dexmedetomidine upregulates microRNA-185 to suppress ovarian cancer growth via inhibiting the SOX9/Wnt/β-catenin signaling pathway
- Source :
- Cell Cycle
- Publication Year :
- 2021
- Publisher :
- Informa UK Limited, 2021.
-
Abstract
- Dexmedetomidine (DEX) could serve as an adjuvant analgesic during cancer therapies. Abnormal expression of microRNAs (miRNAs) could lead to cancer development. This study was aimed to explore the roles of DEX in ovarian cancer (OC) development. OC cell lines SKOV3 and HO-8910 were treated with DEX, after which OC development and the miR-185, SOX9, and Wnt/β-catenin pathway were measured. DEX-treated HO-8910 cells were transfected with miR-185 mimic, miR-185 antisense or miR-185 antisense + silenced SOX9 to further measure the OC cell growth. The target relation between miR-185 and SOX9 was identified, and SOX9 and Wnt/β-catenin pathway were protein levels detected after miR-185 transfection. The role of miR-185 in OC in vivo was also measured. Our study found DEX had a dose-dependent inhibition on OC growth, and DEX promoted miR-185 but suppressed SOX9 expression in OC cells. miR-185 targeted SOX9. After interfering with miR-185 expression, HO-8910 cell proliferation, invasion, migration, and apoptosis were affected. SOX9 knockdown repressed OC development and Wnt/β-catenin pathway. The volume, weight, positive rate of Ki67, CyclinD1, p53 and the degree of tumor necrosis were affected by miR-185 expression. This study demonstrated that DEX could inhibit OC development via upregulating miR-185 expression and inactivating the SOX9/Wnt/β-catenin signaling pathway.
- Subjects :
- Male
0301 basic medicine
endocrine system
Mice, Nude
Biology
Mice
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
microRNA
Adrenergic alpha-2 Receptor Agonists
Animals
Humans
Wnt Signaling Pathway
Molecular Biology
Cell Proliferation
Ovarian Neoplasms
Mice, Inbred BALB C
Gene knockdown
Cell growth
Wnt signaling pathway
SOX9 Transcription Factor
Cell Biology
Transfection
Xenograft Model Antitumor Assays
Up-Regulation
MicroRNAs
030104 developmental biology
Apoptosis
Cell culture
030220 oncology & carcinogenesis
embryonic structures
Cancer research
Female
Signal transduction
Dexmedetomidine
Research Paper
Developmental Biology
Subjects
Details
- ISSN :
- 15514005 and 15384101
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Cell Cycle
- Accession number :
- edsair.doi.dedup.....070ea234850c3930e330370b0bc0b954