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Lack of TRPV2 impairs thermogenesis in mouse brown adipose tissue
Lack of TRPV2 impairs thermogenesis in mouse brown adipose tissue
- Publication Year :
- 2016
- Publisher :
- John Wiley and Sons Inc., 2016.
-
Abstract
- Brown adipose tissue (BAT), a major site for mammalian non-shivering thermogenesis, could be a target for prevention and treatment of human obesity. Transient receptor potential vanilloid 2 (TRPV2), a Ca(2+)-permeable non-selective cation channel, plays vital roles in the regulation of various cellular functions. Here, we show that TRPV2 is expressed in brown adipocytes and that mRNA levels of thermogenic genes are reduced in both cultured brown adipocytes and BAT from TRPV2 knockout (TRPV2KO) mice. The induction of thermogenic genes in response to β-adrenergic receptor stimulation is also decreased in TRPV2KO brown adipocytes and suppressed by reduced intracellular Ca(2+) concentrations in wild-type brown adipocytes. In addition, TRPV2KO mice have more white adipose tissue and larger brown adipocytes and show cold intolerance, and lower BAT temperature increases in response to β-adrenergic receptor stimulation. Furthermore, TRPV2KO mice have increased body weight and fat upon high-fat-diet treatment. Based on these findings, we conclude that TRPV2 has a role in BAT thermogenesis and could be a target for human obesity therapy.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Autocommentary
TRPV2
Adipose tissue
TRPV Cation Channels
White adipose tissue
Biology
Diet, High-Fat
Biochemistry
03 medical and health sciences
Transient receptor potential channel
Mice
0302 clinical medicine
Adipose Tissue, Brown
Internal medicine
Receptors, Adrenergic, beta
Brown adipose tissue
Genetics
medicine
Animals
Obesity
Molecular Biology
Cells, Cultured
PRDM16
Voltage-dependent calcium channel
Cell Differentiation
Thermogenesis
Articles
Mice, Inbred C57BL
Adipocytes, Brown
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Calcium
Calcium Channels
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....0720c603f4fbb1a3b11945948e2058e2