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Synthesis and characterization of strontium-substituted hydroxyapatite nanoparticles for bone regeneration
- Source :
- Materials Science and Engineering: C. 71:653-662
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- The production of stable suspensions of strontium-substituted hydroxyapatite (Sr-HA) nanopowders, as Sr ions vector for bone tissue regeneration, was carried out in the present work. Sr-HA nanopowders were synthesized via aqueous precipitation methods using Sr2+ amount from 0 to 100mol% and were characterized by several complementary techniques such as solid-state Nuclear Magnetic Resonance spectroscopy, X-ray diffraction, Infrared spectroscopy, N2 physisorption and Transmission Electron Microscopy. The substitution of Ca2+ with Sr2+ in HA is always isomorphic with gradual evolution between the two limit compositions (containing 100% Ca and 100% Sr), this pointing out the homogeneity of the synthesized nanopowders and the complete solubility of strontium in HA lattice. Strontium addition is responsible for an increasing c/a ratio in the triclinic unit cell. A significant variation of the nanopowders shape and dimension is also observed, a preferential growth along the c-axis direction being evident at higher strontium loads. Modifications in the local chemical environment of phosphate and hydroxyl groups in the apatite lattice are also observed. Stable suspensions were produced by dispersing the synthesized nanopowders in bovine serum albumin. Characterization by Dynamic Light Scattering and ζ-potential determination allowed to show that Ca2+→Sr2+ substitution influences the hydrodynamic diameter, which is always twice the particles size determined by TEM, the nanoparticles being always negatively charged as a result from the albumin rearrangement upon the interaction with nanoparticles surface. The biocompatibility of the suspensions was studied in terms of cell viability, apoptosis, proliferation and morphology, using osteosarcoma cell line SAOS-2. The data pointed out an increased cell proliferation for HA nanoparticles containing larger Sr2+ load, the cells morphology remaining essentially unaffected.
- Subjects :
- inorganic chemicals
Bone Regeneration
Materials science
Cell Survival
Infrared spectroscopy
chemistry.chemical_element
Nanoparticle
Mineralogy
Apoptosis
Bioengineering
02 engineering and technology
010402 general chemistry
01 natural sciences
Apatite
Biomaterials
Suspensions
Dynamic light scattering
Solid state 31P NMR
Cell Line, Tumor
Strontium hydroxyapatite
Animals
Humans
Bone regeneration
Cell Proliferation
Nanoparticles
Osteoblast
Materials Science (all)
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering
Strontium
Aqueous solution
021001 nanoscience & nanotechnology
0104 chemical sciences
Durapatite
chemistry
Transmission electron microscopy
visual_art
visual_art.visual_art_medium
Cattle
0210 nano-technology
Nuclear chemistry
Subjects
Details
- ISSN :
- 09284931
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Materials Science and Engineering: C
- Accession number :
- edsair.doi.dedup.....07272d2003264054d9bef4d604f6d379