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Preliminary profiling of blood transcriptome in a rat model of hemorrhagic shock
- Source :
- Experimental biology and medicine (Maywood, N.J.), vol 242, iss 14
- Publication Year :
- 2017
- Publisher :
- SAGE Publications, 2017.
-
Abstract
- Hemorrhagic shock is a leading cause of morbidity and mortality worldwide. Significant blood loss may lead to decreased blood pressure and inadequate tissue perfusion with resultant organ failure and death, even after replacement of lost blood volume. One reason for this high acuity is that the fundamental mechanisms of shock are poorly understood. Proteomic and metabolomic approaches have been used to investigate the molecular events occurring in hemorrhagic shock but, to our knowledge, a systematic analysis of the transcriptomic profile is missing. Therefore, a pilot analysis using paired-end RNA sequencing was used to identify changes that occur in the blood transcriptome of rats subjected to hemorrhagic shock after blood reinfusion. Hemorrhagic shock was induced using a Wigger’s shock model. The transcriptome of whole blood from shocked animals shows modulation of genes related to inflammation and immune response (Tlr13, Il1b, Ccl6, Lgals3), antioxidant functions (Mt2A, Mt1), tissue injury and repair pathways (Gpnmb, Trim72) and lipid mediators (Alox5ap, Ltb4r, Ptger2) compared with control animals. These findings are congruent with results obtained in hemorrhagic shock analysis by other authors using metabolomics and proteomics. The analysis of blood transcriptome may be a valuable tool to understand the biological changes occurring in hemorrhagic shock and a promising approach for the identification of novel biomarkers and therapeutic targets. Impact statement This study provides the first pilot analysis of the changes occurring in transcriptome expression of whole blood in hemorrhagic shock (HS) rats. We showed that the analysis of blood transcriptome is a useful approach to investigate pathways and functional alterations in this disease condition. This pilot study encourages the possible application of transcriptome analysis in the clinical setting, for the molecular profiling of whole blood in HS patients.
- Subjects :
- Male
Genetics and Molecular Biology (all)
0301 basic medicine
Biochemistry & Molecular Biology
hemorrhagic shock
Rats
reperfusion
RNAseq
transcriptome
whole blood
Animals
Blood Cells
Disease Models, Animal
Rats, Wistar
Sequence Analysis, RNA
Shock, Hemorrhagic
Gene Expression Profiling
Biochemistry, Genetics and Molecular Biology (all)
Wistar
Inflammation
Blood volume
Biology
Proteomics
Bioinformatics
Medical and Health Sciences
Biochemistry
General Biochemistry, Genetics and Molecular Biology
Transcriptome
03 medical and health sciences
Metabolomics
Genetics
medicine
2.1 Biological and endogenous factors
Aetiology
Hemorrhagic
Original Research
Whole blood
Animal
Human Genome
Shock
Gene expression profiling
Good Health and Well Being
030104 developmental biology
Decreased blood pressure
Disease Models
RNA
medicine.symptom
Sequence Analysis
Biotechnology
Subjects
Details
- ISSN :
- 15353699 and 15353702
- Volume :
- 242
- Database :
- OpenAIRE
- Journal :
- Experimental Biology and Medicine
- Accession number :
- edsair.doi.dedup.....072ad5722495d6a41763f4fcd94e4306