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Yinzhihuang attenuates ANIT-induced intrahepatic cholestasis in rats through upregulation of Mrp2 and Bsep expressions
- Source :
- Pediatric Research. 79:589-595
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- The treatment of intrahepatic cholestasis has been limited, and development of an effective drug is needed. Clinical studies have shown that Yinzhihuang (YZH), a traditional Chinese decoction, enhances bilirubin clearance. The goal of this study was to determine the protective effect of YZH on experimental intrahepatic cholestasis in young rats and to explore its underlying molecular mechanisms. Intrahepatic cholestasis in rats was induced by α-naphthylisothiocyanate (ANIT) on days 1 and 8. The rats received YZH, ursodeoxycholic acid (UDCA), or vehicle for 9 d and were killed on either day 3 or day 10. Serum biomarkers, liver histology, and the distribution of protein and mRNA expression of Mrp2 and Bsep were analyzed. YZH treatment resulted in decreased levels of serum biomarkers except γ-glutamyl transpeptidase, attenuated liver histological injuries, increased protein expressions of Mrp2 and Bsep, and upregulated expressions of Mrp2 and Bsep mRNAs. The effects of YZH on serum biomarkers (aminotransferase, alanine aminotransferase, and direct bilirubin), liver histology, and Mrp2 mRNA expressions were significantly greater and earlier than those of UDCA. Our results suggest that YZH has protective effect against ANIT-induced intrahepatic cholestasis in rats, through upregulation of Mrp2 and Bsep expressions.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Bilirubin
Cholestasis, Intrahepatic
03 medical and health sciences
chemistry.chemical_compound
Downregulation and upregulation
Cholestasis
1-Naphthylisothiocyanate
Internal medicine
medicine
Animals
Distribution (pharmacology)
RNA, Messenger
ATP Binding Cassette Transporter, Subfamily B, Member 11
Messenger RNA
business.industry
Multidrug resistance-associated protein 2
medicine.disease
Multidrug Resistance-Associated Protein 2
Ursodeoxycholic acid
Rats
Up-Regulation
030104 developmental biology
Endocrinology
Liver
chemistry
Pediatrics, Perinatology and Child Health
ATP-Binding Cassette Transporters
Multidrug Resistance-Associated Proteins
business
Biomarkers
Drugs, Chinese Herbal
medicine.drug
Subjects
Details
- ISSN :
- 15300447 and 00313998
- Volume :
- 79
- Database :
- OpenAIRE
- Journal :
- Pediatric Research
- Accession number :
- edsair.doi.dedup.....0736aa26b906180a027660fed71d4d7a