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MiR-302b regulates cell functions and acts as a potential biomarker to predict recurrence in bladder cancer
- Source :
- Life sciences. 209
- Publication Year :
- 2018
-
Abstract
- Background Bladder cancer is the most common urogenital tumor with substantial morbidity, high recurrence rate and mortality. miRNAs, a class of endogenous noncoding RNA, were found to involve in the genesis, maintenance and metastasis of cancer. Genomic profiling revealed that miR-302b is down-regulated in bladder cancer while its functions in bladder cancer remain to be ascertained. Methods Cell functional assays including wound healing assay, CCK-8 assay, Transwell assay and flow cytometry assay were performed to clarify the functions of miR-302b expression in cell proliferation, migration, invasion and apoptosis in BC. Furthermore, RT-qPCR was performed to study the expression of miR-302b in bladder cancer tissues and the prognostic value of altered miR-302b expression with 48 formalin-fixed paraffin-embedded bladder urothelial carcinoma samples. Results The results of RT-qPCR demonstrated that expression level of miR-302b was significantly reduced in bladder cancer tissues and cell lines. The cells after transfected with miR-302b mimic showed lower mobility, lower proliferation and increased apoptosis, while opposite results were obtained after inhibiting the expression of miR-302b. The prognosis analysis demonstrated that the patients with low expression of miR-302b experienced high risks of recurrence. Conclusions The results of our study demonstrate that miR-302b regulates cell functions and acts as a potential biomarker to predict recurrence in bladder cancer.
- Subjects :
- 0301 basic medicine
Male
Cell
Apoptosis
General Biochemistry, Genetics and Molecular Biology
Metastasis
Flow cytometry
03 medical and health sciences
0302 clinical medicine
Cell Movement
microRNA
medicine
Biomarkers, Tumor
Humans
General Pharmacology, Toxicology and Pharmaceutics
Cell Proliferation
Bladder cancer
medicine.diagnostic_test
business.industry
Cancer
General Medicine
Middle Aged
medicine.disease
Prognosis
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
Urinary Bladder Neoplasms
030220 oncology & carcinogenesis
Cancer research
Biomarker (medicine)
Female
Neoplasm Recurrence, Local
business
Subjects
Details
- ISSN :
- 18790631
- Volume :
- 209
- Database :
- OpenAIRE
- Journal :
- Life sciences
- Accession number :
- edsair.doi.dedup.....0739e499cd6455f923a2a9347ecb0fd6