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Mediator subunit MED25 links the jasmonate receptor to transcriptionally active chromatin
- Publication Year :
- 2017
- Publisher :
- National Academy of Sciences, 2017.
-
Abstract
- Jasmonoyl-isoleucine (JA-Ile), the active form of the plant hormone jasmonate (JA), is sensed by the F-box protein CORONATINE INSENSITIVE 1 (COI1), a component of a functional Skp-Cullin-F-box E3 ubiquitin ligase complex. Sensing of JA-Ile by COI1 rapidly triggers genome-wide transcriptional changes that are largely regulated by the basic helix-loop-helix transcription factor MYC2. However, it remains unclear how the JA-Ile receptor protein COI1 relays hormone-specific regulatory signals to the RNA polymerase II general transcriptional machinery. Here, we report that the plant transcriptional coactivator complex Mediator directly links COI1 to the promoters of MYC2 target genes. MED25, a subunit of the Mediator complex, brings COI1 to MYC2 target promoters and facilitates COI1-dependent degradation of jasmonate-ZIM domain (JAZ) transcriptional repressors. MED25 and COI1 influence each other's enrichment on MYC2 target promoters. Furthermore, MED25 physically and functionally interacts with HISTONE ACETYLTRANSFERASE1 (HAC1), which plays an important role in JA signaling by selectively regulating histone (H) 3 lysine (K) 9 (H3K9) acetylation of MYC2 target promoters. Moreover, the enrichment and function of HAC1 on MYC2 target promoters depend on COI1 and MED25. Therefore, the MED25 interface of Mediator links COI1 with HAC1-dependent H3K9 acetylation to activate MYC2-regulated transcription of JA-responsive genes. This study exemplifies how a single Mediator subunit integrates the actions of both genetic and epigenetic regulators into a concerted transcriptional program.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Arsenate Reductases
Arabidopsis
RNA polymerase II
Cyclopentanes
Biology
01 natural sciences
MED1
Histones
03 medical and health sciences
Mediator
Gene Expression Regulation, Plant
Tobacco
Oxylipins
Promoter Regions, Genetic
Transcription factor
Transcriptionally active chromatin
Genetics
Multidisciplinary
Arabidopsis Proteins
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Lysine
Nuclear Proteins
Promoter
Acetylation
Plants, Genetically Modified
Chromatin
Cell biology
Ubiquitin ligase
DNA-Binding Proteins
Repressor Proteins
030104 developmental biology
Nuclear receptor
PNAS Plus
biology.protein
Co-Repressor Proteins
010606 plant biology & botany
Peptide Termination Factors
Signal Transduction
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....075b0d047753fcbf990d0eb813789376