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Nobiletin Enhances the Development and Quality of Bovine Embryos In Vitro During Two Key Periods of Embryonic Genome Activation
- Source :
- Addi. Archivo Digital para la Docencia y la Investigación, instname, Scientific Reports, Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP, Addi: Archivo Digital para la Docencia y la Investigación, Universidad del País Vasco, Scientific Reports, Vol 11, Iss 1, Pp 1-18 (2021), Digital.CSIC. Repositorio Institucional del CSIC
- Publication Year :
- 2021
- Publisher :
- Nature, 2021.
-
Abstract
- In vitro culture can alter the development and quality of bovine embryos. Therefore, we aimed to evaluate whether nobiletin supplementation during EGA improves embryonic development and blastocyst quality and if it affects PI3K/AKT signaling pathway. In vitro zygotes were cultured in SOF + 5% FCS (Control) or supplemented with 5, 10 or 25 µM nobiletin (Nob5, Nob10, Nob25) or with 0.03% dimethyl-sulfoxide (CDMSO) during minor (2 to 8-cell stage; MNEGA) or major (8 to 16-cell stage; MJEGA) EGA phase. Blastocyst yield on Day 8 was higher in Nob5 (42.7 ± 1.0%) and Nob10 (44.4 ± 1.3%) for MNEGA phase and in Nob10 (61.0 ± 0.8%) for MJEGA phase compared to other groups. Mitochondrial activity was higher and lipid content was reduced in blastocysts produced with nobiletin, irrespective of EGA phase. The mRNA abundance of CDK2, H3-3B, H3-3A, GPX1, NFE2L2 and PPARα transcripts was increased in 8-cells, 16-cells and blastocysts from nobiletin groups. Immunofluorescence analysis revealed immunoreactive proteins for p-AKT forms (Thr308 and Ser473) in bovine blastocysts produced with nobiletin. In conclusion, nobiletin supplementation during EGA has a positive effect on preimplantation bovine embryonic development in vitro and corroborates on the quality improvement of the produced blastocysts which could be modulated by the activation of AKT signaling pathway.<br />This work was funded by the Spanish Ministry of Science and Innovation (PID2019-111641RB-I00 to D.R. and RTI2018-093548-B-I00 to A.G.-A). Y.N.C. was supported by a predoctoral fellowship from the Secretaría Nacional de Educación Superior, Ciencia, Tecnología e Innovación (Convocatoria abierta 2017, SENESCYT-Ecuador). C.L.V.L. was supported by a BPE grant from Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil (FAPESP #2017/20339-3).
- Subjects :
- 0301 basic medicine
Signaling pathways
Molecular biology
Nobiletin
Embryo Culture Techniques
chemistry.chemical_compound
Phosphatidylinositol 3-Kinases
0302 clinical medicine
Zygotic transition
Pregnancy
Gene-expression
Phosphorylation
030219 obstetrics & reproductive medicine
Multidisciplinary
Zygote
Chemistry
Gene Expression Regulation, Developmental
BIOLOGIA MOLECULAR
Mitochondria
medicine.anatomical_structure
preimplantation development
Medicine
Female
Signal Transduction
Biotechnology
Development linking
Science
Embryonic Development
zygotic transition
Fertilization in Vitro
Article
Andrology
03 medical and health sciences
Oocyte maturation
Developmental biology
medicine
Animals
Blastocyst
PI3K/AKT/mTOR pathway
Akt/PKB signaling pathway
AKT
Embryogenesis
blastocyst development
Flavones
Cell-cycle
Embryonic stem cell
gene-expression
In vitro
signaling pathways
Preimplantation development
Metabolism
030104 developmental biology
oocyte maturation
cell-cycle
Cattle
evelopment linking
metabolism
Biomarkers
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Addi. Archivo Digital para la Docencia y la Investigación, instname, Scientific Reports, Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP, Addi: Archivo Digital para la Docencia y la Investigación, Universidad del País Vasco, Scientific Reports, Vol 11, Iss 1, Pp 1-18 (2021), Digital.CSIC. Repositorio Institucional del CSIC
- Accession number :
- edsair.doi.dedup.....076006d7d23991256e4deb998cbbdb1b