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Species identification of polygonati rhizoma in China by both morphological and molecular marker methods
- Source :
- Comptes Rendus Biologies. 341:102-110
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Morphological markers as well as two types of molecular markers, inter-sample sequence repeat (ISSR) and start codon targeted (SCoT) are suitable for species identification of the polygonati rhizoma germplasms. In this paper, we adopted these methods for the identification of rhizomes collected from 47 areas in China. Based on their morphological characters, the collected germplasms were classified into two populations, one with alternate leaf arrangement and the other with verticillate leaf arrangement, and they were comprised of five species and fourteen subgroups. Of the five species identified: Polygonatum kingianum, P. cirrhifolium, P. alternicirrhosum, and P. sibiricum belonged to one cluster, and P. cyrtonema belonged to a different cluster. According to the analysis of both ISSR and SCoT markers, all germplasms with greater genetic similarity were classified into one group. Especially, P. sibiricum and P. cirrhifolium, which shared ∼80% similarity, were clustered together, whereas the germplasms identified as P. kingianum with ∼86% similarity formed a separate clade. P. kingianum showed a much greater genetic similarity with P. cyrtonema than with P. sibiricum. The multidimensional scaling analysis further verified the accuracy and reliability of the molecular marker-based results. Thus, both morphological and molecular methods should be combined for the differentiation of germplasms such as those of polygonati rhizoma.
- Subjects :
- Genetic Markers
0106 biological sciences
0301 basic medicine
China
Biology
01 natural sciences
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
chemistry.chemical_compound
Start codon
Similarity (network science)
Molecular marker
Multidimensional scaling analysis
Species identification
Clade
Phylogeny
General Immunology and Microbiology
Polygonatum
Genetic Variation
Reproducibility of Results
General Medicine
Plant Leaves
030104 developmental biology
chemistry
Evolutionary biology
Identification (biology)
General Agricultural and Biological Sciences
Rhizome
010606 plant biology & botany
Polygonatum kingianum
Subjects
Details
- ISSN :
- 16310691
- Volume :
- 341
- Database :
- OpenAIRE
- Journal :
- Comptes Rendus Biologies
- Accession number :
- edsair.doi.dedup.....07670b9f81734ba8820745b064d5adfb