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Perturbation of biogenesis and targeting of Epstein–Barr virus-encoded miR-BART3 microRNA by adenosine-to-inosine editing
- Source :
- Journal of General Virology. 94:2739-2744
- Publication Year :
- 2013
- Publisher :
- Microbiology Society, 2013.
-
Abstract
- Epstein–Barr virus (EBV) encodes at least 44 mature microRNAs (miRNAs), some of which are abundantly expressed in nasopharyngeal carcinoma cells. EBV-encoded miR-BART6 miRNA is known to undergo adenosine-to-inosine (A-to-I) RNA editing, which impacts on processing and function. Whether additional EBV miRNAs might be A-to-I edited remains to be determined. In this study, we have reported on A-to-I editing of EBV miR-BART3. The A-to-I editing enzyme was expressed abundantly in EBV-infected epithelial carcinoma cells. pri-miR-BART3 was found to be edited at four sites in these cells and in nasopharyngeal carcinoma samples. Whereas editing of the second site located within the seed region prevented the targeting of DICE1 mRNA, editing of the third site effectively crippled the biogenesis of mature miR-BART3. Thus, A-to-I editing perturbs biogenesis and targeting of miR-BART3 and may contribute to its differential expression and function in EBV-infected epithelial cells.
- Subjects :
- Epstein-Barr Virus Infections
Herpesvirus 4, Human
Adenosine
Molecular Sequence Data
Biology
medicine.disease_cause
Virus
Cell Line
hemic and lymphatic diseases
Virology
microRNA
medicine
Humans
Inosine
Messenger RNA
Nasopharyngeal Carcinoma
Base Sequence
Carcinoma
Nasopharyngeal Neoplasms
medicine.disease
Molecular biology
Epstein–Barr virus
MicroRNAs
HEK293 Cells
Nasopharyngeal carcinoma
RNA editing
RNA Editing
Carrier Proteins
Biogenesis
Transcription Factors
medicine.drug
Subjects
Details
- ISSN :
- 14652099 and 00221317
- Volume :
- 94
- Database :
- OpenAIRE
- Journal :
- Journal of General Virology
- Accession number :
- edsair.doi.dedup.....076a3e4cd167df54b74571c761497cd2